Chemical discrimination is important for reptiles in reducing population competition and mating choice. In order to understand the discriminating ability of Crocodile Lizard(Shinisaurus crocodilurus)on familiar and unfamiliar individual through chemical cues,we used cotton swabs containing perfume(cologne),chemical stimuli from urine of familiar and unfamiliar individuals as chemical odours,to test the discrimination ability of lizard by counting the times of tongue flicking and latency duration. Both of male and female Crocodile Lizards flicked their tongue much higher frequency when they were served cotton swabs with familiar and unfamiliar odours than they did with cologne;the latency time toward familiar and unfamiliar odours were significantly shorter than that toward cologne. However,the tongue-flick frequency of males toward unfamiliar and familiar male odour was not significant difference,but males exhibited a much shorter latency time toward the former than they did toward the latter. Males licked their tongue more frequency at shorter latency time when they tested unfamiliar female odours than they did with familiar female odour. Female responded to familiar male odour was significantly shorter than they did to unfamiliar male odour. Male licked their tongue with a significant higher frequency in responding to unfamiliar female odour than female did to unfamiliar male odour. The results showed that the Crocodile Lizard could discriminate conspecific chemical cues from control cues,and could discriminate familiar and unfamiliar ones from chemical cues. This ability of discrimination familiar and unfamiliar ones may play important role in holding territory and in finding mating chance.