| 刘坤,苏广华,段彪,魏著英,吴侠,李光鹏.2012.藏羚羊成纤维细胞系的建立及其核型分析.动物学杂志,47(1):16-24. |
| 藏羚羊成纤维细胞系的建立及其核型分析 |
| Culture of the Fibroblast Cells and Their Karyotype Analyses in Pantholops hodgsonii |
| 投稿时间：2011-07-18 修订日期：2011-11-09 |
| DOI： |
| 中文关键词: 藏羚羊 生长曲线 染色体 核型 |
| 英文关键词:Pantholops hodgsonii Growth curve Chromosome Karyotype |
| 基金项目:国家高新技术发展计划(863)项目(No.2008AA10Z159),内蒙古大学生殖生物学及生物技术教育部创新团队项目 |
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| 中文摘要: |
| 藏羚羊(Pantholops hodgsonii)是我国特有的国家Ⅰ级重点保护野生动物,生存在高海拔低氧环境下,具有高原生物的细胞遗传特性。实验采用组织块贴壁培养法进行原代培养,后经差异贴壁法联合消化排除法纯化,成功构建了藏羚羊成纤维细胞系,并对培养不同代次细胞的形态、生长动力学、染色体核型等进行了研究。结果表明,培养得到的藏羚羊细胞为典型的成纤维细胞,第7、10、15代细胞群体倍增时间分别为26、48、50 h。细胞的生长均为"S"型。染色体中二倍体染色体(2n=60)占主体,所占比例为65%～91%,包括性染色体X、Y在内均为端着丝粒类型,染色体组的总相对长度为193.45,平均相对长度为3.22,未发现随体、次缢痕等特殊标志性特征。 |
| 英文摘要: |
| Pantholops hodgsonii is a unique wild animal species in China,and it is listed as an Appendix species by CITES and as Category Ⅰ by the Key Protected Wildlife List of China.Individuals of P.hodgsonii live in high altitude and low oxygen environment,with typical genetic characteristics of plateau animals.The P.hodgsoni fibroblast cells derived from a piece of ear margin tissue were successfully cultured and then purified by differential attachment in combination with digestion elimination methods.The morphology,dynamic growth,and karyotypes of the cultured cells at different passages were analyzed.The results showed that the cultured cells had typical characteristics of fibroblasts.The population doubling times (PDT) of passage 7,10 and 15 were 26 h,48 h,and 50 h,respectively.The growth curve of the cultured cells appeared "S" shape,and diploid cells (2n=60) were dominant (65%-91%).All of the chromosomes were telocentric (including X and Y chromosomes).The total and average relative length of chromosome set was 193.45 and 3.22, respectively.No satellites and secondary constriction were observed in the present study. |
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