| 何志新,张方.2011.应用不同固定及染色方法确定商城肥鲵肥大细胞的分布.动物学杂志,46(3):69-77. |
| 应用不同固定及染色方法确定商城肥鲵肥大细胞的分布 |
| Applying Different Fixatives and Staining Methods to Ascertain Distribution of Mast Cells in Pachyhynobius shangchengensis |
| 投稿时间：2010-11-14 修订日期：2011-03-03 |
| DOI： |
| 中文关键词: 商城肥鲵 肥大细胞 组织化学 形态学 |
| 英文关键词:Pachyhynobius shangchengensis Mast cell Histochemistry Morphology |
| 基金项目:安徽师范大学博士启动基金项目(No.160-750817),重要生物资源的保护及利用安徽省重点实验室基金资助; |
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| 中文摘要: |
| 为了解商城肥鲵( Pachyhynobius shangchengensis )肥大细胞(mast cell, MC)的分布和形态特点,丰富有尾两栖类MC的生物学资料,用不同固定和染色方法对商城肥鲵消化系统及其他部分器官的肥大细胞进行了观察,并对消化道和舌的MC数量进行统计。结果表明,除输卵管和肾外,在3种固定方法中,改良甲苯胺蓝染色法(MTB)在其他器官中均能显示出MC,而阿利新蓝-藏红染色法(AB-S)仅在消化道中能显示出AB+和S+两种MC,其余各器官只能显示AB+细胞。MC呈圆形、椭圆形、长梭形、长条形、不规则形,有围绕血管和腺体分布的倾向,在舌、食道、胃、皮肤、输卵管和子宫结合部分布较多,肠系膜、肝、胰、肌肉、子宫、小肠、十二指肠、直肠分布较少。在MTB染色中,肥大细胞胞浆颗粒呈紫红色、红色、蓝紫色,轮廓清晰,与背景色(蓝色)对比性强,而在AB-S染色中,MC显蓝色(消化道中有少量红染MC存在),背景淡染,对比性不如前者。本研究发现,MC在商城肥鲵绝大部分器官组织中存在,甲苯胺蓝染色法是显示商城肥鲵MC的优良方法。 |
| 英文摘要: |
| To investigate the distributive pattern and morphological characteristics of mast cells (MCs) in Pachyhynobius shangchengensis , MCs from digestive system and some of other organs were observed by using different fixatives and staining methods, then cell numbers in digestive tract and tongue were counted and analyzed statistically. The results indicated that improved toluidine blue staining (MTB) could be used to display MCs in all organs except for the Fallopian tube and kidney in 3 fixatives, but Alcian blue-Safranin O staining (AB-S) displayed AB+ MCs and S+MCs in digestive tract and only AB+ MCs in other organs. The shape of MCs was round, ellipse, long-shuttle, linear or irregular. The MCs tended to be adjacent to blood vessels and glands. Numerous MCs were found in tongue, esophagus, stomach, skin, the combining site of uterus and the Fallopian tube, and a few in mesentery, liver, pancreas, muscle, uterus, small intestine, duodenum and rectum. Evidently, different from background color (blue), MC plasma in MTB was purple, red, bluish violet and the outline of MCs could be observed clearly, whereas MCs in AB-S was blue (a few red MCs in digestive tract), with the background poorly stained. These data show that MCs exist in most tissues of P.shangchengensis , and that toluidine blue better stains mast cells. |
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