| 王茜,姜美玲,武玉珑,张志明,王兰.2010.苯酚对多刺裸腹溞糖及蛋白质代谢酶的影响.动物学杂志,45(6):78-84. |
| 苯酚对多刺裸腹溞糖及蛋白质代谢酶的影响 |
| Effects of Phenol on the Carbohydrate and Protein Metabolism Enzymes of Moina macrocopa |
| 投稿时间：2010-03-22 修订日期：2010-07-08 |
| DOI： |
| 中文关键词: 多刺裸腹溞 代谢酶 苯酚 |
| 英文关键词:Moina macrocopa Metabolism enzymes Phenol |
| 基金项目:山西省青年自然科学基金项目(No.20051035) |
|
| 摘要点击次数: 2346 |
| 全文下载次数: 2345 |
| 中文摘要: |
| 采用急性毒性方法,研究了苯酚对多刺裸腹溞(Moina macrocopa)糖及蛋白质代谢的影响。实验设对照组和5个苯酚处理组,苯酚浓度分别为0.25、0.75、1.25、1.75、2.25 mg/L。分别在苯酚处理24 h和48 h后用分光光度法测定丙酮酸激酶(PK)、乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、谷草转氨酶(GOT)和谷丙转氨酶(GPT)的活力。结果显示,随着苯酚浓度的增加和处理时间的延长,LDH活力呈现升高趋势,PK和SDH活力与对照组相比没有显著性差异。GOT活力在处理24 h后先升后降,处理48 h后活力降低;GPT活力在处理24 h、48 h后活力均升高。结论是:苯酚中毒需要高水平的代谢能量,导致裸腹溞代谢发生重大改变,诱导能量代谢一定程度上由碳水化合物分解代谢向蛋白分解代谢转变。 |
| 英文摘要: |
| To explore the effects of phenol on the carbohydrate and protein metabolism,Moina macrocopa were exposed to phenol(0.25,0.75,1.25,1.75,2.25 mg /L) for 24 and 48 hours.Activities of several key metabolic enzymes including pyruvate kinase(PK),lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT) were investigated.The results showed that the activity of LDH was gradually increased following the increase of phenol concentration and treatment duration.PK and SDH activities remained almost unchanged.After 24 h,the activity of GOT firstly showed increase,and then decrease.After 48 h,the activity of GOT decreased.The activity of GPT was slightly elevated,but there was no significant difference when compared with controls.All these results indicate that phenol intoxication demands high level metabolic energy and leads to significant changes of the metabolic profile of the daphnia,inducing to a certain extent a shift from carbohydrate catabolism to protein catabolism.The LDH may serve as a biomarker of aquatic animal for assessing the biochemical toxicity of phenolic contamination. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
