| 肖羽,陈光照,谷晓明.2010.贵州疣螈D-loop区和tRNAPhe序列变异及群体遗传多样性.动物学杂志,45(3):21-29. |
| 贵州疣螈D-loop区和tRNAPhe序列变异及群体遗传多样性 |
| Sequence Variation of Mitochondrial DNA D-loop including tRNAPhe and Population Genetic Diversity of Tylototriton kweichowensis |
| 修订日期：2010-03-02 |
| DOI： |
| 中文关键词: 贵州疣螈 D-loop区 tRNAPhe基因 遗传多样性 基因流 |
| 英文关键词:Tylototriton kweichowensis mtDNA D-loop tRNAPhe gene Genetic diversity Gene flow |
| 基金项目:贵州省教育厅自然科学研究项目(No.黔教科(2007)017);贵州省国际科技合作重点项目(No.黔科合外G字(2008)700116) |
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| 中文摘要: |
| 贵州疣螈(Tylototritonkweichowensis)是我国特有的国家Ⅱ级保护动物,分布面积极为狭窄。为了解其遗传多样性现状,作者采获了几乎覆盖其分布范围的12群体的58个体的材料,测定了线粒体D-loop区(mtDNA控制区)及tRNAPhe基因全序列共808bp,发现16个变异位点,定义了26个单元型。遗传多样性检测显示,贵州疣螈种群具有高的单元型多样性和低的核苷酸多样性。Tajima’sD和FuandLi’sD值检测结果均表明,贵州疣螈种群相对于中性进化的歧异度并没有明显的偏离。系统发育及网络进化分析没有显示出单元型和地理位置的对应关系,各群体的单元型都相互散布在不同的分布群中,呈现一种混杂的分布格局。水城群体与其他任一群体间基因流Nm<1,有分化的迹象。总的来说,贵州疣螈各群体之间没有发生极显著的遗传分化,因此建议将贵州疣螈所有群体作为一个整体管理单元加以保护。 |
| 英文摘要: |
| Tylototriton kweichowensis is endemic to China and is listed as a category Ⅱ species of the state key protected wildlife. Its distribution area is extremely limited. To study the genetic diversity and its genetic variation of T. kweichowensis populations,we sequenced the complete mitochondrial DNA D-loop ( mtDNA control region) and tRNAPhe of 58 samples obtained from 12 populations. Only 16 variable sites were detected in the nucleotide sequences of 808 bp,and 26 haplotypes were identified. T. kweichowensis populations have high haplotype diversity and low nucleotide diversity. Neither the estimate of Tajima’s D nor that of Fu and Li’s D deviated significantly from the neutral selection hypothesis for 12 populations. Phylogenetic trees and Median joining Network analysis demonstrated that all the haplotypes could not be clustered to corresponding geographic clades,but showed disordered distribution pattern. There are signs of genetic divergence between Shuicheng population and anyone of others,according to gene flow Nm < 1. In general,there is no distinctive geneticdivergence among its populations. |
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