| 付晶,白秀娟.2010.鸵鸟性别鉴定的分子标记方法.动物学杂志,45(2):82-87. |
| 鸵鸟性别鉴定的分子标记方法 |
| A Molecular Method for Sex Identification in the Ostrich (Struthio camelus) |
| 投稿时间：2009-08-05 修订日期：2009-12-23 |
| DOI： |
| 中文关键词: 平胸鸟类 鸵鸟 性别鉴定 EE0.6 CHD |
| 英文关键词:Ratite bird Ostrich (Struthio camelus) Sex identification EE0. 6 CHD |
| 基金项目: |
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| 中文摘要: |
| 鸵鸟(Struthiocamelus)属于平胸总目鸟类,雌雄鸵鸟在性成熟前外部形态相同,很难通过外观和形态来鉴定性别,给早期分群饲养造成了很大的困难。实验利用鸵鸟羽毛提取基因组DNA,之后利用EE0.6和CHD基因中2个引物组合对3对已知性别和9只未知性别鸵鸟的性别基因片段进行特异性扩增。结果显示,这对引物组合在雄性鸵鸟的DNA中未扩增出片段,在雌性鸵鸟DNA中扩增出1条片段,可以对鸵鸟的性别作出准确鉴定,从而解决幼雏期鸵鸟难以从外貌上区分其性别的问题。 |
| 英文摘要: |
| It is difficult to identify the sex of Ostrich ( Struthio camelus) with morphological method before reaching sexual maturity,and this situation challenged the early feeding of Ostrich. In this study,genome DNA was extracted from feathers by using nondestructive sampling method and a combination of primer from one of EE0. 6 and the other of CHD. Sex gene sequences were amplified and compared among three pairs of sex-known birds and 9 sex-unknown birds. The results showed that male birds displayed no distinct amplification band, whereas one band was amplified for female ones. Thus the method could be used for sexing Ostrich before sexual maturity. |
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