川金丝猴mtDNA D-loop序列遗传多态性分析
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国家“十一五”科技支撑计划项目(No.2006BAD03A1202,2007BAC03A08-5);国家科技基础条件平台建设项目(No.2005DKA21404)


Analysis on Mitochondrial DNA D-loop Sequences Genetic Polymorphism of Rhinopithecus roxellana
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    摘要:

    采用非损伤性DNA分析技术,分析了甘肃白水江保护区、陕西长青保护区、湖北神农架保护区3个川金丝猴(Rhinopithecus roxellana)种群中的20份粪便样品和2份肌肉样品,成功扩增了mtDNA D-loop区部分片段。经过GenBank数据库的BLAST比对,确定了22份样品均来自川金丝猴,经过Clustal W和DNASP软件分析,在22份川金丝猴mtDNA D-loop区393bp中,共检测出54个多态性位点,分为17个单倍型,单倍型多态性(h)为0.965,核苷酸多态性(π)为3.10%。3个种群之间的遗传距离为0.003~0.098,核苷酸差异为0.08%~2.80%,表明所得到的川金丝猴样品中存在着较丰富的遗传多态性,种群间存在一定的遗传差异。

    Abstract:

    By using noninvasive DNA technology,20 fecal samples and 2 muscle samples of Sichuan Snub-nosed Monkeys(Rhinopithecus roxellana)from Baishuijiang Nature Reserve of Gansu province(BSJ),Changqing Nature Reserve of Shaanxi Province(SH) and Shennongjia Nature Reserve of Hubei Province(SNJ) were analyzed and the mitochondrial DNA D-loop fragments were successfully amplified.Sequences were aligned with the GenBank by BLAST software and 22 samples were identified as R.roxellana.According to the analysis by Clustal W and DNASP software,17 haplotypes and 54 diversity sites were found in 22 Sichuan snub-nosed monkey samples with 393 bp length of mtDNA D-loop region.The haplotype diversity was 0.965 and nucleotide diversity was 3.10%.The genetic distance and nucleotide divergence of R.roxellana among BSJ,SH,and SNJ were 0.003-0.098 and 0.08%-2.80%,respectively.Therefore,the results show a high variation in the mitochondrial DNA D-loop region and genetic distance of R.roxellana from the three sampling sites.

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何丽,张于光,李迪强,李大全.2010.川金丝猴mtDNA D-loop序列遗传多态性分析.动物学杂志,45(1):70-76.

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  • 收稿日期:2009-08-06
  • 最后修改日期:2009-10-29
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