| 王悦,俞诗源,高先军,王昱,郭婷婷.2009.Ghrelin、KGF、TGF-β(β2、β3)在大鵟胃肠道中的表达.动物学杂志,44(6):122-129. |
| Ghrelin、KGF、TGF-β(β2、β3)在大鵟胃肠道中的表达 |
| Expression of Ghrelin,KGF and TGF-β (β2,β3) in Gastrointestinal Tract of Buteo hemilasius |
| 投稿时间：2009-07-20 修订日期：2009-09-25 |
| DOI： |
| 中文关键词: 大鵟 胃肠道 生长素 角质细胞生长因子 转化生长因子β 免疫组织化学 |
| 英文关键词:Buteo hemilasius Gastrointestinal tract Ghrelin KGF TGF-β Immunohistochemistry |
| 基金项目:国家自然科学基金项目(No.30370163);甘肃省创新人才基金项目(No.ZS006-016) |
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| 中文摘要: |
| 应用免疫组织化学方法和体视学半定量方法,检测了ghrelin、KGF、TGF-β(TGF-β2,TGF-β3)在大鵟(Buteo hemilasius)胃肠道中的表达,利用IPP专业图像分析软件对其表达强度进行了定量分析。结果表明,ghrelin免疫反应阳性物质分布在十二指肠、空肠、回肠、盲肠和直肠的黏膜层,主要分布于黏膜上皮、肠腺上皮和固有层。从十二指肠到盲肠阳性细胞的分布密度逐渐减小,直肠阳性细胞的分布密度高于盲肠。胃肠道呈KGF免疫反应阳性,胃内阳性物质分布于腺胃浅腺和深腺、肌胃黏膜、肌胃单管腺的上皮细胞;肠内阳性物质分布于固有层的血管、淋巴和平滑肌纤维。胃黏膜、腺胃深腺、肌胃单管腺、肠道黏膜、肠腺呈TGF-β2及TGF-β3免疫反应阳性,阳性物质分布于黏膜、肠腺上皮细胞胞质中。图像分析显示,KGF的阳性表达水平呈波浪形变化,在空肠和直肠处达到峰值;TGF-β2的阳性表达水平呈波浪形变化,分别在肌胃和空肠处有峰值;TGF-β3从腺胃到空肠阳性表达水平逐渐增强,之后阳性表达水平又逐渐下降,到盲肠阳性表达水平回升。ghrelin、KGF、TGF-β2和TGF-β3的阳性表达强弱可能与胃肠道的消化能力有关,它们的协同表达调控鸟类胃肠道的生长和发育。 |
| 英文摘要: |
| The expression of ghrelin,KGF,TGF-β2 and TGF-β3 in gastrointestinal tract of Buteo hemilasius was investigated by immunohistochemistry combined with stereological methods,and the expression intensity of these growth factors was analyzed by IPP professional image analysis software. Results showed that ghrelin immunopositive cells mainly distributed in the mucosal layer of all intestine segments:duodenum,jejunum,ileum,caecum and rectum. The ghrelin immunopositive cell density gradually decreased from the duodenum to the caecum,and its distribution density in the rectum was higher than that in the caecum. Gastrointestinal tract showed positive KGF immunoreactivity. KGF immunopositive cells mainly distributed in the epithelial cells of the superficial glands and deep glands of glandular stomach mucosa,as well as mucosa,simple tubular glands of muscular stomach. KGF immunoreactive cells were also found in the blood vessel,lymph and smooth muscle fiber of the intestine lamina propia. TGF-β2 and TGF-β3 immunopositive cells were found in the mucosa,and deep glands of glandular stomach,simple tubular glands of muscular stomach,intestine,and intestinal glands. They mainly distributed in the cytoplasm of the epithelial cells. Analysis of images showed that the KGF displayed a wave-like distribution pattern in the gastrointestinal tract,with the maximal distribution in the jejunum and rectum. The TGF-β2 also displayed a wave-like distribution pattern in the gastrointestinal tract,with the maximal expression in the muscular stomach and jejunum. The expression of TGF-β3 increased from glandular stomach to jejunum,decreased progressively after jejunum,and increased again in caecum. The expression of ghrelin,KGF,TGF-β2 and TGF-β3 may be closely related with the digestive function of gastrointestinal tract. Their coordinated expression may control the growth and development of gastrointestinal tract in birds. |
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