| 郭婷婷,俞诗源,高先军,王悦,王昱.2009.大(狂鸟)和长耳鸮的部分血液生理生化指标及血细胞的显微结构观察.动物学杂志,44(3):94-100. |
| 大(狂鸟)和长耳鸮的部分血液生理生化指标及血细胞的显微结构观察 |
| The Hemogram and Biochemical/Physiological Indices of the Blood in Buteo hemilasius and Asio otus |
| 投稿时间：2008-10-15 修订日期：2009-03-05 |
| DOI： |
| 中文关键词: 大(狂鸟) 长耳鸮 血象 生理生化指标 血细胞 显微结构 |
| 英文关键词:Buteo hemilasius Asio otus Hemogram Physiological and biochemical indices Blood cell Microstructure |
| 基金项目:国家自然科学基金项目(No.30370163);甘肃省自然科学基金项目(No.ZS031-A25-005-Z);甘肃省创新人才基金项目 |
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| 中文摘要: |
| 为了解大(狂鸟)(Buteohemilasius)、长耳鸮(Asiootus)的血象、血液生理生化参数正常值及血细胞的形态特征,为其健康检测和保护提供参考资料,采用纽巴氏法、沙利氏比色法、离心法、瑞士染色等方法检测了大(狂鸟)和长耳鸮的血象,利用全自动血液生物化学分析仪测定了两种鸟的19项血液生化指标,利用生物显微技术对其血细胞进行观察。结果表明:大(狂鸟)的红细胞数量、红细胞压积、红细胞分布宽度、血红蛋白含量、平均血红蛋白浓度、嗜酸性粒细胞数量、淋巴细胞数量、白细胞数量及凝血细胞数量均高于长耳鸮,而其他指标均低于长耳鸮;其中,红细胞平均体积在两种鸟类间存在极显著性差异(P<0.01),其他均无显著性差异(P>0.05)。在生理生化检测中,大(狂鸟)的谷草转氨酶、总蛋白含量、白蛋白含量、球蛋白含量、总胆固醇、甘油三酯和葡萄糖含量均高于长耳鸮,且大(狂鸟)的无机离子中的钠和氯离子浓度也高于长耳鸮,而其他指标均低于长耳鸮,其中谷丙转氨酶和肌酐具有极显著性差异(P<0.01),尿酸和葡萄糖具有显著性差异(P<0.05),其他指标均无显著性差异(P>0.05)。大(狂鸟)和长耳鸮的血细胞形态相似。种属不同及生活习性的差异可能是导致这两种鸟类血液生理常值差别及显微结构差异的主要原因,而采样时的机体状况、营养状况及测试操作方法也有一定的影响。 |
| 英文摘要: |
| In this study,19 physiological and biochemical indices of the blood in Buteo hemilasius and Asio otus were determined by adopting modified Neubauer method,Sahlis colorimetric method,centrifugal method,Wright’s staining method as well as automatic biochemical analyzer.Results showed that the erythrocyte number,hematocrit,erythrocyte hemoglobin distribution width,hemoglobin content,mean corpuscular hemoglobin concentration,acidophilic granulocyte number,lymphocyte number,white blood cell number and thrombocyte number of B.hemilasius were higher than those of A.otus,while other indices were lower.There was an extremely significant difference in the mean corpuscular volume between B.hemilasius and A.otus(P<0.01).The glutamic-oxalacetic transaminase,total protein,albumin,globulin,glucose,plasma natrium and chlorine,as well as the total cholesterol and triglyceride of the B.hemilasius were higher than those of A.otus,while other indices were lower.Significant difference and extremely significant difference were observed in the content of glutamate-pyruvate transaminase,creatinine,uric acid and glucose between B.hemilasius and A.otus(P<0.01 or P<0.05),but no significant difference was found in the other indices(P>0.05).Although the shapes of blood cells of B.hemilasius were similar to those of A.otus,differences were also observed.The differences in the physiological and biochemical values as well as the sizes of the blood cells can be attributed to their different species,and also to their different living habits,body conditions,nutrition conditions and to some extent to the determination methods. |
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