| 丁建文,庄平,章龙珍,侯俊利,张涛,刘鉴毅,颜世伟.2009.西伯利亚鲟卵黄脂磷蛋白的分离纯化及性质.动物学杂志,44(3):9-15. |
| 西伯利亚鲟卵黄脂磷蛋白的分离纯化及性质 |
| Purification and Partial Characterization of Lipovitellin from Acipenser baerii |
| 投稿时间：2008-11-26 修订日期：2009-03-01 |
| DOI： |
| 中文关键词: 西伯利亚鲟 卵黄脂磷蛋白 凝胶过滤层析 氨基酸 |
| 英文关键词:Acipenser baeri) Lipovitellin Gel filtration chromatography Amino acids |
| 基金项目:国家“863”计划项目(No.2008AA10Z227);农业科技成果转化基金项目(No.2008GB23260408);浙江省科技计划项目(No.2007C12026);中央级公益性科研院所基本科研业务费专项资金资助项目(No.2007M01,2007M023) |
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| 中文摘要: |
| 卵黄是鱼类胚胎发生期的主要营养物质,卵黄的含量和质量对于早期幼体维持生命和生长发育至关重要。本研究采用Sephacryl S-300凝胶过滤层析法和蛋白质电泳技术分离纯化西伯利亚鲟(Acipenser baerii)卵黄脂磷蛋白(lipovitellin,Lv),层析洗脱共得到7个蛋白峰。对每个峰进行SDS-PAGE电泳及油红O、甲基绿和Schiff试剂特异染色,峰b蛋白均呈阳性,表明峰b蛋白为西伯利亚鲟卵中的一种卵黄脂磷蛋白,SDS-PAGE电泳分析表明,其由3个亚基构成,相对分子质量分别为30.6ku、40.8ku和76.7ku。对西伯利亚鲟Lv氨基酸组成进行分析,证明是一种含有相对较多天冬氨酸、赖氨酸、谷氨酸、丝氨酸、缬氨酸和亮氨酸的蛋白,并且所含鲜味氨基酸含量比其他鱼偏高。 |
| 英文摘要: |
| The yolk protein is the chief nutrition material during embryogenesis.The quantity and quality of yolk is crucial for early larval growth.We have used Sephacryl S-300 gel filtration chromatography to separate lipovitellin from the Acipenser baerii.There were seven protein chromatography peaks.The peak b protein was composed of three subunits with molecular weights of 30.6 ku,40.8 ku and 76.7 ku,respectively as revealed by the SDS-PAGE electrophoresis analysis.Oil red O,Methyl green and the Schiff staining showed that the peak b protein was rich in glucose,lipid and phosphorus.Lipovitellin of A.baerii contained rich asparagines acid,lysine acid,glutamic acid,serine acid,leucine acid and valine acid.The quantity of flavor amino acids such as glutamic acid and asparagines acid in A.baerii was abundant when compared to that in other species. |
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