| 霍金龙,苗永旺,霍海龙,李大林,刘丽仙,刘建平,李云辉.2008.云南南部地区野猪群体的遗传多样性.动物学杂志,43(6):137-146. |
| 云南南部地区野猪群体的遗传多样性 |
| Genetic Diversity of Sus scrofa in Southern Area of Yunnan Province Based on Microsatellite Markers |
| 投稿时间：2008-02-28 修订日期：2008-09-08 |
| DOI： |
| 中文关键词: 野猪 微卫星标记 遗传多样性 杂合度 多态信息含量 |
| 英文关键词:Wild Boar Microsatellite DNA Genetic diversity Heterozygosity Polymorphism information content |
| 基金项目:云南省教育厅科学研究基金项目(No.07Y40124);国家自然科学基金项目(No.30660024);云南省应用基础研究重点项目(No.2007C0003Z);云南省应用基础研究计划面上项目(No.2006C0034M) |
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| 中文摘要: |
| 野猪(Sus scrofa)是家猪的祖先,也是野生动物遗传资源和生物多样性的重要组成部分。为了阐明其群体遗传变异,以对其进一步有效保护和合理利用提供科学依据,本研究采用分布在家猪19条染色体上的76个微卫星标记对云南南部地区野猪群体65只个体进行了群体遗传变异分析。共检测到349个等位基因,每个座位的等位基因数从3到9不等,有效等位基因数在1.6835～8.0667之间,平均每个座位等位基因数(4.5921±1.1452)个,有效等位基因数(3.7099±0.9904)个,群体平均表观杂合度、期望杂合度及平均多态信息含量分别为(0.9492±0.1135)、(0.7116±0.0787)和(0.6634±0.0921)。结果表明,本实验检测到的野猪群体遗传多样性较丰富。 |
| 英文摘要: |
| Wild Boar (Sus scrofa) is not only the ancestor of domestic pigs,but also important genetic resources and the constituent of biological diversity.In order to further get insight into the genetic variability in its population and provide the genetic background and guidance for its conservation and utilization,the microsatellite DNA polymorphisms for 65 individuals sampled from the southern area of Yunnan province were assayed by using PCR and polyacrylamide gel electrophoresis.Of 76 microsatellite loci tested which are located on swine's 19 chromosomes,a total of 349 alleles were detected,and the number of alleles varied from 3 to 9 and the number of effective alleles from 1.683 5 to 8.066 7, with the mean value of 4.592 1±1.145 2 alleles and 3.709 9±0.990 4 effective alleles per locus.The observed heterozygosity,expected heterozygosity and polymorphism information content were estimated to be 0.949 2±0.113 5,0.711 6±0.078 7,and 0.663 4±0.092 1,respectively.The results show that the genetic diversity in the population of the Wild Boar in Yunnan Province is high. |
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