Endosulfan is a kind of manufactured organochlorine pesticide.It is widely used to control insects in agriculture,including food crops such as grains,vegetables,fruits,and tea.Although endosulfan plays an important role in controlling insects and increasing crop production,it affects aquatic organism survival.The primary objective of the research provided information of aquatic toxicology and evaluated the survival risks of aquatic organism during acute and sub-chronic endosulfan exposure.The paper summarized the toxicological effects of endosulfan on Zebrafish(Danio rerio) in glass aquaria under static conditions.The results showed that the concentrations of endosulfan that killed 50% of the Zebrafish within 24 h,48 h,72 h,96 h LC₅₀ were 4.24 μg/L,2.49 μg/L,1.77 μg/L,1.62 μg/L respectively.After being exposed to sub-lethal concentrations of endosulfan for 30 d,significant effects of endosulfan on superoxide dismutase(SOD) and acetylcholinesterases(AChE) activity of Zebrafish had been found.The activity of SOD in livers and brains for experimental groups was significantly increased comparing with control groups.The activity of AChE in livers and brains was decreased in dose of endosulfan 0.17 μg/L and 0.74 μg/L.