Institute of Biology Science & Technology,Donghua University,Shanghai 201600; Joint Laboratory of Model Animal Biodiversity,Shanghai 201200,China 在知网中查找 在百度中查找 在本站中查找
Institute of Biology Science & Technology,Donghua University,Shanghai 201600; Joint Laboratory of Model Animal Biodiversity,Shanghai 201200,China 在知网中查找 在百度中查找 在本站中查找
This study established a multiplex STR genotyping system through universal fluorescent primers in combination with sequencing.For each panel of multiplex STR genotyping amplification,two sets of primers were used,namely universal primers labeled with FAM and specific primers with an added 5' tail.We optimized the proportion of universal and specific primers and reaction of multiplex PCR was achieved.By using this method,five STR marker loci genotyping were successfully conducted within a single reaction.The optimized universal fluorescent primers amplification genotyping system can be used in multiplex STR genotyping,which allows reliable,effective and low-cost genotyping compared with regular microsatellite fluorescent detection assays.
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