| 陈国亮,邵爱云,孟清.2007.一种提取蜘蛛基因组DNA的有效方法.动物学杂志,42(6):64-68. |
| 一种提取蜘蛛基因组DNA的有效方法 |
| A Efficient Method for Extraction of Spiders’ Genomic DNA |
| 投稿时间：2007-07-05 修订日期：2007-09-17 |
| DOI： |
| 中文关键词: 蜘蛛 基因组 DNA DNA 提取 PCR 扩增 |
| 英文关键词:Spider Genomic DNA DNA extraction PCR amplification |
| 基金项目:国家高技术研究发展计划(863计划)资助项目(No.2006AA03Z0451); |
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| 中文摘要: |
| 介绍了用尿素法提取蜘蛛基因组 DNA。通过与其他 DNA 提取方法相比较,证明尿素法具有可在室温条件下进行、DNA 得率高、完整性好、简单快速等优点。以提取的DNA为模板进行 PCR 扩增,获得预期大小的、高重复、高GC含量的编码蜘蛛牵引丝蛋白基因的 DNA 片段。 |
| 英文摘要: |
| The present paper deals with the urea lyses method for isolating spiders’ genomic DNAs.Compared to other two known methods,this method produced spider genomic DNAs with higher yields and higher integrity.It is also simpler and less time-consuming,involving only room temperature manipulations.High quality genomic DNAs were isolated from spider by this method.Certain spider dragline gene fragments,which contain highly repetitive sequences of high GC contents,were successfully PCR-amplified. |
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