| 曹海鹏,杨先乐,王玉洁,李圆圆.2007.鲟源致病性豚鼠气单胞菌的分离及其生长特性.动物学杂志,42(6):1-6. |
| 鲟源致病性豚鼠气单胞菌的分离及其生长特性 |
| Isolation and Growth Characteristics of Pathogenic Aeromonas punctata caviae from Sturgeons |
| 投稿时间：2007-05-15 修订日期：2007-09-19 |
| DOI： |
| 中文关键词: 杂交鲟 达氏鳇 细菌性败血综合征 豚鼠气单胞菌 生长特性 |
| 英文关键词:Huso huso(♀)×Acipenser ruthenus(♂) H. dauricus Bacterial septicaemia syndrome Aeromonas punctata caviae Growth characteristics |
| 基金项目:国家科技支撑计划资助项目(No.006BAD03B0506),上海市重点学科建设资助项目(Y1101),上海市教育委员会E-研究院建设资助项目(E03009); |
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| 中文摘要: |
| 从患细菌性败血综合征的杂交鲟(Huso huso ♀× Acipenser ruthenus ♂)肝和达氏鳇(H. dauricus)腹水中共分离出4株优势菌,经人工回归感染实验测定了其致病性,并通过ATB细菌鉴定仪对致病菌株进行了鉴定,此外,研究了致病菌株的生长特性。实验结果表明,菌株XL2-T对杂交鲟和达氏鳇具有致病性。经鉴定,菌株XL2-T为豚鼠气单胞菌(Aeromonas punctata caviae);其在无菌营养肉汤中摇床振荡培养时的生长曲线:0~1.5 h为生长延迟期,1.5~28 h为对数生长期,28~32 h为稳定期,32 h以后为衰亡期;其最适生长温度为25~30℃,最适生长pH为7,在NaCl含量范围0~10%、沙拉沙星浓度范围0~40 μg/ml下均能够生长,但NaCl、沙拉沙星对其生长具有较大的抑制作用。 |
| 英文摘要: |
| Four strains of domain bacteria were isolated from the liver of Hybrid Sturgeons,Huso huso(♀)×Acipenser ruthenu (♂) and ascites of Kaluga Sturgeons,H. dauricus.Their pathogenity was determined by artificial infection test.The pathogenic strain was identified by ATB bacteria identification instrument,and its growth characteristics was also studied.The results indicated that,strain XL2-T was pathogenic to Hybrid Sturgeons and Kaluga Sturgeons,and was identified as Aeromonas punctata caviae.Its growth curve in the sterile liquid culture medium was as follows:lag phase 0-1.5 h, log phase 1.5-28 h,stationary phase 28-32 h, and decline phase after 32 h.Its best growth conditions were 25-30℃ and pH 7,and it could grow in culture medium with 0-10% NaCl and 0-40 μg/ml sarafloxacin,but NaCl and sarafloxacin had some inhibitory effects on its growth. |
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