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白玉婷,杨默言#,刘新勇,薛梦琦,季珂萌,王晓丽.2020.非特异性酯酶及酸性和碱性磷酸酶 在奥尼罗非鱼消化道的分布.动物学杂志,55(3):387-392.
非特异性酯酶及酸性和碱性磷酸酶 在奥尼罗非鱼消化道的分布
Distribution of Nonspecific Esterase, Acid Phosphatase and Alkaline Phosphatase in Digestive Tract of Tilapia Galilaea
投稿时间:2019-11-07  修订日期:2020-04-27
DOI:10.13859/j.cjz.202003013
中文关键词:  奥尼罗非鱼  消化道  非特异性酯酶  酸性磷酸酶  碱性磷酸酶
英文关键词:Tilapia Galilaea (Oreochromis niloticus ♀ × O. aureus ♂)  Digestive tract  Nonspecific esterase  Acid phosphatase  Alkaline phosphatase
基金项目:
作者单位E-mail
白玉婷 广西大学动物科学技术学院 南宁 530005 931995968@qq.com 
杨默言# 广西大学动物科学技术学院 南宁 530005 675637363@qq.com 
刘新勇 广西大学动物科学技术学院 南宁 530005 349478746@qq.com 
薛梦琦 广西大学动物科学技术学院 南宁 530005 1030475470@qq.com 
季珂萌 广西大学动物科学技术学院 南宁 530005 jkm1019@qq.com 
王晓丽 广西大学动物科学技术学院 南宁 530005 xiaoliwang@gxu.edu.cn 
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中文摘要:
      本研究利用石蜡切片结合组织化学方法研究奥尼罗非鱼(Oreochromis niloticus ♀ × O. aureus ♂)消化道内非特异性酯酶、酸性磷酸酶和碱性磷酸酶的分布特点。结果发现,非特异性酯酶大量密集分布在前肠,其次为胃、食管和中肠,极少数零散分布在后肠。酸性磷酸酶主要分布于奥尼罗非鱼前肠,其次为中肠和胃,极少数分布在食管和后肠。碱性磷酸酶大量密集分布在奥尼罗非鱼前肠和中肠,其次为胃、食管和后肠。结果表明,奥尼罗非鱼前肠、中肠、食管和胃有较强的吸收和消化脂质功能,前肠、中肠和胃是蛋白质吸收和消化的主要部位,前肠和中肠是营养物质吸收的主要部位。
英文摘要:
      The distributions of nonspecific esterase, acid phosphatase and alkaline phosphatase in digestive tract of Tilapia Galilaea (Oreochromis niloticus ♀ × O. aureus ♂) were studied by paraffin section combined with histochemical methods including Gomori’s staining and acid-α-naphthyl-acetate esterase staining. It was found that nonspecific esterase densely distributed in the foregut, massively distributed in the stomach, esophagus and midgut, and slightly distributed in the hindgut (Fig. 1). Acid phosphatase mainly distributed in the foregut, followed by the midgut and stomach, and few in the esophagus and hindgut (Fig. 2). Alkaline phosphatase was densely observed in the foregut and midgut, and a larger amount was also observed in the stomach, esophagus and hindgut (Fig. 3). The results showed that the foregut, midgut, esophagus and stomach of Tilapia Galilaea were the main sites of lipid absorption and digestion. The foregut, midgut and stomach were the main sites of protein absorption and digestion, and the foregut and midgut were the main sites of nutrient absorption.
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