• 首页关于本刊期刊订阅编委会作者指南过刊浏览
王瑛莹.2020.华南虎血常规两种测定方法的比较应用.动物学杂志,55(2):264-271.
华南虎血常规两种测定方法的比较应用
Comparative Application of Two Methods for Determination of Blood Routine in South China Tiger
投稿时间:2019-09-25  修订日期:2020-03-17
DOI:10.13859/j.cjz.202002016
中文关键词:  华南虎  血常规  全自动血液分析仪  人工显微镜法
英文关键词:The South China tiger  CBC  Automated hematology analyzer  Manual microscope method
基金项目:上海市绿化和市容管理局资助项目
作者单位E-mail
王瑛莹 上海动物园 329846444@qq.com 
摘要点击次数: 51
全文下载次数: 44
中文摘要:
      通过全自动血细胞分析仪与人工镜检法分别对华南虎(Panthera tigris amoyensis)血常规进行分析,并对获得结果做比较分析,探究建立适合华南虎血细胞分析的方法。收集临床华南虎血液标本40份,分别使用全自动血液分析仪和人工显微镜检法对红细胞、白细胞进行计数检测,并对白细胞进行分类分析。如人工计数和仪器计数获得的数据均符合正态分布,则应用配对样本T检验比较差异性,否则应用非参数检验(两个相关样本:Wilcoxon带符号秩检验)。相关性采用Spearman分析,以P < 0.05为差异有统计学意义。结果表明,通过两种方法获得的红细胞计数、白细胞计数、中性粒细胞比率、嗜酸性粒细胞比率结果差异均不显著(P > 0.05);淋巴细胞比率、单核细胞比率和嗜碱性粒细胞比率,两种方法检测结果具有显著性差异(P < 0.05)。红细胞计数(r = 0.915)、白细胞计数(r = 0.832)、淋巴细胞比率(r = 0.832)、中性粒细胞比率(r = 0.481)应用两种方法获得的结果相关性均较好。单核细胞比率(r = 0.283)、嗜酸性粒细胞比率(r = 0.309)、嗜碱性粒细胞比率(r = 0.146)两种检测方法相关系数都低于0.4,且P值接近或大于0.05,呈弱相关或极弱相关。全自动血细胞分析仪可准确测定华南虎红细胞、白细胞数量,计数具有准确、快速、可靠的优点;然而,该法不适用于白细胞分类计数,必要时人工与仪器结合方可保证检验结果的准确性。本研究为实现华南虎血细胞快速准确分析提供参考。
英文摘要:
      The complete blood count (CBC) of the South China tiger was analyzed by automated hematology analyzer and manual microscope method respectively in this study. By comparing the results obtained from the two methods, a suitable method for CBC of South China tiger was explored. Forty blood samples from South China tiger were collected and examined. When data detected by the two methods were normally distributed, paired sample T-test was used to analyze the difference, otherwise, Wilcoxon signed rank test was used. Correlation analysis was performed by Spearman analysis and the statistical significance was judged when P value was less than 0.05. The results showed that there was no significant difference for the red blood cells counts (P > 0.05), white blood cells counts (P > 0.05), neutrophil ratio (P > 0.05), eosinophil ratio (P > 0.05) between the two methods. And there were significant differences in the lymphocyte ratio (P < 0.05), monocyte ratio (P < 0.05) and basophil ratio (P < 0.05). The two sets of data were well correlated in red blood cells counts (r = 0.915), white blood cells counts (r = 0.832), lymphocyte ratio (r = 0.832), and neutrophil ratio (r = 0.481), while the correlation coefficients of monocyte ratio (r = 0.283), eosinophil ratio (r = 0.309) and basophil ratio (r = 0.146) were lower than 0.4, and P value was greater than or equal to 0.05, showing weak or extremely weak correlation. Thus, the automatic hematology analyzer can be used to accurately detect the count of both red blood cells and white blood cells of South China tiger, which has the advantages of accuracy, rapidity and reliability. However, this method is not suitable for the classification of white blood cells. If necessary, the accuracy of the results can be guaranteed by combining microscope and machine method. This study provides a reference for the rapid and accurate analysis of blood cells in South China tiger.
附件
查看全文  查看/发表评论  下载PDF阅读器