| 王毅花,陈丽霞,马天,杨亮亮,孙戈,钱法文,郑常明.2020.笼养黑颈鹤粪便性激素水平的时间动态.动物学杂志,55(3):363-370. |
| 笼养黑颈鹤粪便性激素水平的时间动态 |
| Physiological Characteristics of Caged Black-necked Cranes during Breeding Season |
| 投稿时间：2019-06-24 修订日期：2020-04-30 |
| DOI：10.13859/j.cjz.202003010 |
| 中文关键词: 黑颈鹤 粪便 性激素 皮质酮 |
| 英文关键词:Black-necked Crane Faeces Gonadal hormone Corticosterone |
| 基金项目:中国林业科学研究院基本科研业务费专项（No. CAFYBB2017QA009） |
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| 中文摘要: |
| 黑颈鹤（Grus nigricollis）为国家Ⅰ级重点保护野生动物，由于其生存的自然环境恶劣，野外种群数量少，许多动物园以及保护区等机构用迁地保护的方式对黑颈鹤进行保护和繁育，然而长期以来笼养黑颈鹤繁殖率低。本研究运用非损伤的研究方法，并结合行为观察，对繁殖期黑颈鹤的繁殖生理特征进行了探索研究。结果表明，进入繁殖期黑颈鹤的雌二醇水平显著升高，可作为有效监测雌性黑颈鹤繁殖期生理状态的重要生理指标之一；繁殖前期雄性黑颈鹤的睾酮水平显著升高，与非繁殖期以及繁殖期的其他阶段差异显著，表明睾酮可有效监测雄性黑颈鹤的繁殖生理状态，为人工采精选择合适的时间提供理论基础；交配成功的雌性黑颈鹤繁殖中期的孕酮水平极显著高于其他阶段，表明该指标可有效监测雌性黑颈鹤的交配状态。综上，通过非损伤性的采样研究手段，遴选合适的生理指标参数可以为黑颈鹤的人工繁育提供理论依据。 |
| 英文摘要: |
| Black-necked Crane (Grus nigricollis) is a national first-class protected wildlife. Due to its harsh natural environment and small population size in the wild, the Black-necked Cranes have been bred in captivity in many zoos and protected areas for protection, however, in low reproduction rate. In order to improve its reproduction rate, we explored the physiological characteristics of Black-necked Crane in captivity during breeding period using non-invasive sampling methods and behavioral observations. The enzyme-linked immunity was used to measure the contents of sex hormones (estradiol, progesterone and testosterone). SPSS was used to conduct one-way ANOVA and multiple comparisons on the data. The significance level was set as P = 0.05. The homogeneity of sample mean variance was determined by Levene’s homogeneity of variance test, Dunnett''s T3 test was used for multiple comparisons of data from different sampling periods. The results showed that: The estradiol level of Black-necked Crane in the breeding period was significantly increased, which could be used as one of the important physiological indicators to effectively monitor the physiological status of females in the breeding period (Fig. 1); The testosterone level of males in the early stage of reproduction was significantly increased, which was significantly different from that in the non-breeding period and other stages of breeding period, indicating that testosterone could effectively monitor the reproductive physiological state of males and provide a theoretical basis for the selection of appropriate time for artificial extraction (Fig. 2 and 4); The progesterone level in the middle reproductive period of the females with successful mating was significantly higher than that in other stages, indicating that this indicator can effectively monitor the mating status of the females (Fig. 1). In conclusion, monitoring the appropriate physiological parameters can provide a theoretical basis for the breeding of captive Black-necked Crane and improve its breeding rate. |
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