马清芝,马波,李雷,金星,林小婉,金洪宇.2019.基于线粒体DNA细胞色素b基因和控制区序列分析西藏雅鲁藏布江黄斑褶鮡种群遗传多样性.动物学杂志,54(5):701-710. |
基于线粒体DNA细胞色素b基因和控制区序列分析西藏雅鲁藏布江黄斑褶鮡种群遗传多样性 |
Analysis of Genetic Diversity of Pseudecheneis sulcata Populations from the Yarlung Zangbo River in Tibet Based on Mitochondrial DNA Cytochrome b Gene and Control Region Sequence |
投稿时间:2019-04-09 修订日期:2019-08-26 |
DOI:10.13859/j.cjz.201905011 |
中文关键词: 黄斑褶鮡 线粒体DNA 细胞色素b基因 D-loop控制区 遗传多样性 雅鲁藏布江 |
英文关键词:Pseudecheneis sulcata Mitochondrial DNA Cyt b D-loop Genetic diversity Yarlung Zangbo River |
基金项目:公益性(农业)行业科研专项(No. 201403012),农业农村部财政专项“西藏重点水域渔业资源与环境调查” |
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中文摘要: |
采用线粒体DNA(mtDNA)Cyt b基因和D-loop控制区为分子标记,对分布于西藏雅鲁藏布江大峡谷以上里龙段和以下墨脱段2个群体的黄斑褶 (Pseudecheneis sulcata)共60个样本进行遗传多样性研究。获得联合基因有效序列长度为1 893 bp,包括Cyt b基因1 060 bp和D-loop控制区833 bp。结果显示,里龙和墨脱2个群体的单倍型多样性值(Hd)均较高(0.701和0.761),核苷酸多样性值(π)均较低(0.001 00和0.001 09);高频率的单倍型Hap1和Hap2为2个群体所共享,推测为祖先单倍型;同时,里龙和墨脱群体分别存在5个和6个特有单倍型,且在2个群体中不共享;分子方差分析(AMOVA)显示遗传变异主要来源于种群内部,群体间呈中度遗传分化水平(Fst = 0.090 44,P < 0.05);中性检验(Tajima''s D、Fu''s Fs)和核苷酸不配对(SSD、Hir)分析结果揭示,黄斑褶 种群曾经历过种群扩张现象。本研究推测,黄斑褶 2个群体间的基因流动存在障碍,雅鲁藏布大峡谷的海拔落差及水文情势等生态屏障可能是阻碍黄斑褶 迁徙和交流的主要原因。 |
英文摘要: |
Using mitochondrial DNA (mtDNA) Cyt b gene and D-loop control region as molecular markers, genetic diversities of 60 Pseudecheneis sulcata samples from two populations in the upper Lilong section and the lower Motuo section of the Yarlung Zangbo River Grand Canyon were studied. The effective sequence length of the combination gene was 1 893 bp, including 1 060 bp Cyt b gene and 833 bp D-loop control region. The results showed that the haplotype diversity values (Hd) of both Lilong and Motuo populations were high (0.701 and 0.761), and the nucleotide diversity values (π) were low (0.001 00 and 0.001 09). A high frequency of Hap1 and Hap2 was observed in both populations, which were presumed to be ancestral haplotypes. Meanwhile, there were 5 and 6 unique haplotypes in Lilong and Motuo populations, respectively, and they were not shared between the two populations. Analysis of molecular variance (AMOVA) showed that the genetic variation mainly originated from within the population, with moderate genetic differentiation between populations (Fst = 0.090 44, P < 0.05). Neutral test (Tajima''s D and Fu''s Fs) and nucleotide mismatch analysis (SSD and Hir) revealed that Pseudecheneis sulcata had experienced population expansion. This study indicated that there were barriers in gene flow between the two populations of P. sulcata. The ecological barriers such as altitude difference and hydrological situation of the Yarlung Zangbo Grand Canyon may be the main reasons for hindering the migration and communication in P. sulcata. |
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