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孟维悦,张国钢,陆军,孟德荣.2019.渤海湾两株H2亚型禽流感病毒的 遗传进化分析.动物学杂志,54(5):736-745.
渤海湾两株H2亚型禽流感病毒的 遗传进化分析
Genetic Evolution of Two Strains of H2 Subtype Avian Influenza Virus Isolated from Bohai Bay
投稿时间:2019-03-04  修订日期:2019-09-09
DOI:10.13859/j.cjz.201905015
中文关键词:  禽流感病毒  H2亚型  遗传进化  鸟类迁徙  渤海湾
英文关键词:Avian influenza virus  H2 subtype  Genetic evolution  Bird migration  Bohai Bay
基金项目:国家林业和草原局野生动物疫病监测和预警系统维护项目
作者单位E-mail
孟维悦 中国林业科学研究院森林生态环境与保护研究所全国鸟类环志中心国家林业和草原局生物多样性重点实验室 北京 100091 mengweiyue13@126.com 
张国钢 中国林业科学研究院森林生态环境与保护研究所全国鸟类环志中心国家林业和草原局生物多样性重点实验室 北京 100091 zm7672@126.com 
陆军 中国林业科学研究院森林生态环境与保护研究所全国鸟类环志中心国家林业和草原局生物多样性重点实验室 北京 100091 lujunmail@vip.sina.com 
孟德荣 沧州师范学院生命科学学院 沧州 061001 mengderong84@163.com 
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中文摘要:
      野鸟是禽流感病毒的自然储存库,病毒可以随着宿主的迁徙传播给其他野鸟与家禽。渤海湾是鸟类南北迁徙的重要停歇地,也是东亚-澳大利西亚鸟类迁徙通道的重要组成部分,每年有大量水鸟在渤海湾停歇,促进了禽流感病毒的传播。为了解渤海湾地区禽流感病毒的传播及进化与水鸟迁徙的相关性,2018年春季鸟类迁徙期间的4和5月份,在渤海湾采集鸻鹬类粪便样品2 120份,对样品进行检测,分离出2株H2亚型禽流感病毒。对这2株H2亚型禽流感病毒进行了分子特征及遗传进化分析,并结合渤海湾水鸟的环志回收数据,对H2亚型病毒的重组及遗传进化与水鸟迁徙的联系进行了分析。结果表明,2株分离株的HA蛋白裂解位点符合低致病性禽流感病毒的分子特征,它们的8个基因片段同源性均不高,其中879-H2N7的8个基因片段分别与我国福建和澳大利亚的毒株同源性最高,遗传关系最近;854-H2N8的8个基因片段分别与我国湖南以及日本、韩国、孟加拉国和越南的毒株同源性最高,遗传关系最近。渤海湾水鸟的环志回收数据分析表明,879-H2N7随着野鸟的迁徙在渤海湾、福建沿海和澳大利亚之间进行传播与扩散;854-H2N8可能跨越东亚-澳大利西亚和中亚-印度两条通道之间进行基因重组和进化,并会随着鸟类迁徙进行传播和扩散。
英文摘要:
      Wild birds are a natural reservoir for avian influenza virus. The virus can spread to other wild birds and domestic birds during migration. Bohai Bay is an important stopover site located in the East Asian-Australasian Flyway. Every year, a large number of waterbirds rest in Bohai Bay, which accelerated the spread of the virus. To understand the correlation between the transmission and evolution of avian influenza virus and waterbirds migration in Bohai Bay during the spring, 2 120 fecal samples of waders were collected in western Bohai Bay from April to May in 2018. The samples were treated and tested, two strains of H2 subtype avian influenza virus were isolated, and their molecular characteristics and genetic evolution were analyzed. Combining the banding and recovery data of waterbirds, the association between the spread of H2 subtype virus and waterbirds migration was explored. The results showed that the HA protein cleavage site of the two isolates were consistent with the molecular characteristics of low pathogenicity avian influenza virus. All 8 gene fragments of these two strains were not highly homologous. The 8 gene fragments of A/waders/Hebei/879/2018 (H2N7) had the highest homology and the closest genetic relationship with the strains in Fujian and Australia (Table1 for homology and Appendix 1 for genetic relationship). The 8 gene fragments of A/waders/Hebei/854/2018 (H2N8) had the highest homology and the closest genetic relationship with the strains in Hunan, Japan, Korea, Bangladesh and Vietnam, respectively (Table 1 for homology and Appendix 2 for genetic relationship). Based on the banding and recovery data of waterbirds in Bohai bay, AIV 879-H2N7 was spread with the wild bird migration between Bohai Bay, Fujian and Australia, AIV 854-H2N8 was spread and genetic reassortment was taken place with wild bird migration between East Asian-Australasian Flyway and Central Asia-India Flyway.
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