We recorded the calls of two male Odorrana tormotus and video-taped their interactions during a territorial combat at Fuxi, Mount Huangshan on 17 April, 2017. The combat lasted about eight minutes, and produced a winner and a loser. The winner emitted 16 calls during the combat and 7 calls after the combat. The loser uttered 5 calls during the combat. At one instant during the territorial encounter, both frogs emitted a call simultaneously. For this study, we used the software Praat to obtain various temporal and spectral parameters, and sheng employed T test to determine whether there was a significant difference in parameter of each call during the combat between two frogs. The winner produced multi-note calls mostly and his calls was with an average fundamental frequency of 8 150 ± 1 305 Hz; the maximum and minimum fundamental frequency was 9 741 ± 1 283 Hz and 5 809 ± 1 139 Hz. The difference between the maximum fundamental frequency and the minimum fundamental frequency was 3 932 ± 1 122 Hz. All the values above was higher than those of the loser′s made, especially on average fundamental frequency and the maximum fundamental frequency (P < 0.05). However, the differences in minimum fundamental frequency and maximum fundamental frequency and the in the minimum fundamental frequency were not significant (P > 0.05). But the dominant frequency produced by the winner was significantly lower than loser′s (P > 0.05). The total call durations of winner and loser were 0.57 ± 0.09 s and 0.51 ± 0.11 s, respectively. The time duration of calls of this species was longer than the duration of advertisement calls of non-combat O. tormota. Comparing to the winner the loser′s calls contained more abundant nonlinear phenomena (NLP); the duration of NLP segments was > 50% longer for the loser than for the winner. The total duration and NLP durarion of combat and post-combat calls for the winner were shorter (P < 0.05) than the loser, the average fundamental frequency was significant decrease (P < 0.05), the maximum fundamental frequency and the minimum fundamental frequency were all lower after winning the combat (P > 0.05), but the break duration was longer. The difference between the maximum fundamental frequency and the minimum fundamental frequency was greater (P > 0.05). It appeared that winner increased the call rate as well as the dominant frequency in order to fight off the opponent. This article described the calling characteristics of male O. tormotus during a territorial combat. It increases our knowledge of signal plasticity in this species.