| 王雪,韩利文,何秋霞,孙晨,张云,王希敏,刘可春.2018.马兜铃酸对斑马鱼肾损伤因子（KIM-1）表达的影响.动物学杂志,53(4):622-627. |
| 马兜铃酸对斑马鱼肾损伤因子（KIM-1）表达的影响 |
| Effect of Aristolochic Acid on Expression of KIM-1 in Zebrafish |
| 投稿时间：2017-09-07 修订日期：2018-05-31 |
| DOI：10.13859/j.cjz.201804013 |
| 中文关键词: 斑马鱼 肾损伤因子（KIM-1） 马兜铃酸 肾毒性 生物标志物 |
| 英文关键词:Zebrafish, Danio rerio Kidney injury molecule (KIM-1) Aristolochic acid Nephrotoxicity Biomarker |
| 基金项目:山东省自主创新重大专项（No. 2014ZZCX0215） |
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| 中文摘要: |
| 采用分子定量方法检测斑马鱼（Danio rerio）幼鱼组织中KIM-1蛋白含量和基因表达水平，了解马兜铃酸处理后斑马鱼体内肾损伤因子的表达变化情况，并与幼鱼表型变化相比较，探讨KIM-1作为肾功能特异标志物在斑马鱼肾功能损伤评价中的应用价值。结果显示，马兜铃酸处理30 h后，幼鱼出现水肿，水肿发生率呈剂量依赖性特点。0.5 ~ 5.0 μmol/L马兜铃酸处理组幼鱼水肿发生率与对照组差异不明显，但幼鱼KIM-1因子的蛋白含量及Kim-1基因表达水平比正常对照组显著升高，在马兜铃酸浓度为2 μmol/L时达到最高。表明在肾功能损伤检测中，KIM-1是一种比表型更早发生变化的指标。 |
| 英文摘要: |
| To explore the application possibility of kidney injury molecule-1 (KIM-1) as a specific biomarker in the evaluation of the renal function, we studied the changes of kidney injury molecule-1 content and its mRNA level in Zebrafish (Danio rerio) after aristolochic acid treatment. The results were compared with the phenotypic changes of Zebrafish. The larvae showed periocular and pericardial edema in a dose-response manner after exposure to aristolochic acid for 30 hours (Table 1). Although the incidence of edema in 0.5﹣5.0 μmol/L aristolochic acid treated groups had no significant difference compared with the control, the protein content and mRNA expression level of KIM-1 in larvae tissue were significantly higher than the control (Table 1, Fig. 1). The peak appeared in the 2 μmol/L aristolochic acid treated group (Fig. 2, 3). From the result we can conclude that KIM-1 is a more sensitive indicator for detection of renal injury, whose changes are earlier than the morphological changes in zebrafish. |
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