| 王喆,汪登强,段辛斌,陈大庆,陈会娟,刘绍平.2018.长江中上游银[鱼句]线粒体DNA遗传多样性分析.动物学杂志,53(4):615-621. |
| 长江中上游银[鱼句]线粒体DNA遗传多样性分析 |
| Genetic Diversity of Squalidus argentatus in the Middle and Upper Reaches of the Yangtze River Inferred from Mitochondrial DNA Sequences |
| 投稿时间：2017-03-28 修订日期：2018-05-09 |
| DOI：10.13859/j.cjz.201804012 |
| 中文关键词: 银[鱼句] 遗传结构 细胞色素b基因 遗传多样性 长江流域 |
| 英文关键词:Squalidus argentatus Genetic structure Cyt b Genetic diversity Yangtze River |
| 基金项目:国家自然科学基金项目（No. 51579247）和农业部物种保护项目长江中上游重要渔业水域主要经济物种产卵场及洄游通道调查项目 |
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| 中文摘要: |
| 银[鱼句]（Squalidus argentatus）是长江常见小型鱼类，分布广泛于干支流，资源量较大。本研究采用线粒体DNA（mtDNA）细胞色素b基因序列对长江中上游干流江津、荆州、监利、洪湖、黄石江段及支流赤水河、湘江等7个江段共217尾银[鱼句]样品的遗传结构和遗传多样性进行了分析。结果显示，银[鱼句]群体Cyt b基因序列分别检出了81个变异位点和97个单倍型，平均单倍型多样性（Hd）和核苷酸多样性（Pi）分别为0.966和0.008 6。分子方差分析（AMOVA）显示，长江中上游银[鱼句]群体存在显著遗传分化。群体间两两比较分析发现，赤水河群体与其他群体基因交流程度低，遗传分化显著，其他群体间没有显著遗传分化。中性检验结果不支持银 历史上发生过群体扩张。 |
| 英文摘要: |
| Squalidus argentatus is a common freshwater fish distributed in Yangtze River, including in the main and tributary river. In this study, the genetic diversity and structure of S. argentatus from the upper and middle Yangtze River were analyzed using mitochondrial DNA Cyt b gene sequence. A total of 217 individuals were collected from 7 sampled sites, including Jiangjin, Jingzhou, Jianli, Honghu and Huangshi in Yangtze River, and Chishui in Chishui River and Yingtian in Xiangjiang River, the two tributaries of Yangtze River. A total of 81 polymorphic sites and 97 haplotypes were detected and the average haplotype diversity (Hd) and average nucleotide diversity (Pi) were 0.966 and 0.008 6, respectively (Table 1). Analysis of molecular variance (AMOVA) suggested that significant genetic differentiation occurred between the Chishui population and the others, and no genetic divergences among the latter six populations were found by pairwise AMOVA analysis (Table 3). The neutral test showed that historical population expansion did not occur in S. argentatus (Fig. 3). |
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