Bdelloidea rotifers are the common invertebrates in moss fauna. However, Bdelloidea rotifers is difficulty to observe due to the smaller size of each in individual and also it is difficulty to separate them. Therefore the research on this group invertebrate was behind compared to other rotifer taxa. This article is aimed at comparing the extraction efficiency on Bdelloidea rotifers by different extraction methods and exploring the affect on extraction efficiency under different experiment condition. The multi-shock resuscitation methods and the sucrose shock resuscitation methods were selected according to the literature reports on extraction methods of moss-dwelling Bdelloidea rotifer. Impact factors, such as pH, temperature and humidity were chosen as different experimental conditions, and the experiment was done by setting up the different experimental groups with Orthogonal table of L9 (Table 1). All the data obtained from the experiment were deal with Excel 2010 and the statistic analysis was processed with SPSS 19.0 for analysis of variance to determine the difference among the experiment results. The study results showed that the average number of Bdelloidea rotifers were 94.68 ind/g and 165.33 ind/g by multi-shock and sucrose shock resuscitation methods, respectively (Fig.1). The sucrose shock resuscitation methods could extract more Bdelloidea rotifers compared with the multi-shock resuscitation methods significantly (F = 24.017, P< 0.05). The influence of the extraction conditions on the extraction efficiency was in the order: pH > humidity > temperature. Both range analysis and variance analysis displayed that while the maximum extraction efficiency can be expected under the following extraction conditions: pH at 7.8, temperature at 20℃ and humidity at 60% respectively (Table 2﹣4).