| 姜宏波,包杰,姜春玖,付佩佩,于业辉,李晓东.2017.pH对中华小长臂虾存活及呼吸代谢的影响.动物学杂志,52(2):322-330. |
| pH对中华小长臂虾存活及呼吸代谢的影响 |
| Effects of pH on Survival and Respiratory Metabolism of Chinese Grass Shrimp (Palaemonetes sinensis) |
| 投稿时间：2016-04-27 修订日期：2016-12-19 |
| DOI：10.13859/j.cjz.201702016 |
| 中文关键词: 中华小长臂虾 pH 耐受性 耗氧率 排氨率 窒息点 |
| 英文关键词:Chinese Grass Shrimp, Palaemonetes sinensis pH Tolerance Oxygen consumption rate Ammonia excretion rate Asphyxiation point |
| 基金项目:辽宁省教育厅科学研究一般项目（No. L2015472，L2014254） |
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| 中文摘要: |
| 为研究中华小长臂虾（Palaemonetes sinensis）对pH的耐受性，采用静水毒性实验方法确定了中华小长臂虾的半致死pH。实验设置酸性条件下的pH为3.0、3.2、3.4、3.6、3.8和4.0，碱性条件下的pH为10.4、10.6、10.8、11.0、11.2和11.4，统计中华小长臂虾在24、48、72和96 h的死亡率。中华小长臂虾24、48、72和96 h的酸性半致死pH（LpH50）分别为3.416、3.426、3.463和3.463，碱性半致死pH（LpH50）分别为10.813、10.609、10.516和10.516，酸性条件下和碱性条件下的安全pH分别为4.463和9.516。在半致死pH（LpH50）基础上以静水密闭式方法研究了pH对中华小长臂虾耗氧率、排氨率和窒息点的影响，pH设置为4、5、6、7、8、9、10，以pH 7为对照组。与对照组相比，只有pH为9和10时的耗氧率显著升高（P < 0.05），其他pH则未发生显著性变化（P > 0.05）；排氨率则只有pH为5和6时显著高于对照组（P < 0.05），其他pH组与对照组没有显著性变化（P > 0.05）；在pH为4 ~ 9时，中华小长臂虾氧氮比（O/N）的变化范围为4.14 ~ 10.95，此时以蛋白质为主要供能物质，随着pH继续上升到10时，中华小长臂虾的氧氮比（O/N）突然增加至29.62，此时供能物质则变成以脂肪为主；在各pH条件下窒息点没有发生显著性变化（P > 0.05）。本实验结果表明，中华小长臂虾对pH有很强的耐受性，但不同的pH会影响到中华小长臂虾的呼吸代谢以及能量供给方式。 |
| 英文摘要: |
| The median lethal pH (LpH50) was investigated for pH tolerance of Chinese Grass Shrimp (Palaemonetes sinensis) by the hydrostatic toxicity experiment method. The acidic range of pH was 3.0, 3.2, 3.4, 3.6, 3.8 and 4.0, and the alkaline range of pH was 10.4, 10.6, 10.8, 11.0, 11.2, and 11.4. The LpH50 in the acidic range was 3.416 for 24 h, 3.426 for 48 h, 3.463 for 72 h and 3.463 for 96 h, respectively (Table 2); The LpH50 in the alkaline range was 10.813 for 24 h, 10.609 for 48 h, 10.516 for 72 h and 10.516 for 96 h, respectively (Table 4). Security pH values were 4.463 and 9.516, respectively. Subsequently, the effects of pH on the oxygen consumption rate, ammonia excretion rate and asphyxiation point in P. sinensis were studied by hydrostatic test based on LpH50. The pH values were set to 4, 5, 6, 7, 8, 9, 10, and pH 7 was control group. We used the software SPSS 18.0 to analyze the experimental data. An analysis of variance (ANOVA) was used to analyze the experimental results, and Duncan was used for multiple comparisons. The results showed that oxygen consumption rates at pH 9 and 10 were significantly increased compared to the control pH (P < 0.05), and other pH groups did not have significantly change (P > 0.05) (Fig. 1); the ammonia excretion rates at pH 5 and 6 were significantly higher compared to the control pH (P < 0.05), and other pH groups did not have significantly change (P > 0.05) (Fig. 2); the range of oxygen-nitrogen ratio (O/N) in P. sinensis was 4.14 ~ 10.95 at pH 4﹣9, indicating that protein was the main energy supply material, however, O/N at pH 10 was sharply increased to 29.62 and the main energy supply material was fat instead of protein (Fig. 3). The asphyxiation point of P. sinensis had no significant difference among different pH groups (Fig. 4). The experimental results show that P. sinensis has strong tolerance to pH, but pH can affect respiratory metabolism and energy supply way in P. sinensis. |
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