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邹远超,李中,李艳,覃川杰,岳兴建,文正勇,王淯,齐泽民,谢碧文.2017.乌鳢三倍体诱导及其生长.动物学杂志,52(1):75-84.
乌鳢三倍体诱导及其生长
Induction and Growth of Triploid Snakehead (Channa argus)
投稿时间:2016-01-19  修订日期:2016-10-12
DOI:10.13859/j.cjz.201701009
中文关键词:  乌鳢  三倍体  诱导  热休克  生长
英文关键词:Snakehead, Channa argus  Triploid  Induction  Heat shock  Growth
基金项目:四川省科技厅应用基础项目(No. 2015JY0262),四川省大学生创新项目(No. 201410640002);
作者单位E-mail
邹远超 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室 zou3891@163.com 
李中 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室  
李艳 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室  
覃川杰 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室  
岳兴建 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室  
文正勇 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室  
王淯 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室  
齐泽民 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室  
谢碧文 内江师范学院生命科学学院长江上游鱼类资源保护与利用四川省重点实验室 576977970@qq.com 
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中文摘要:
      采用热休克抑制第二极体排放的方法诱导乌鳢(Channa argus)三倍体,以探索人工诱导乌鳢三倍体的理想热休克条件。采用DNA含量测定法和红细胞核大小鉴定法对获得的鱼苗倍性进行鉴定,同时对普通二倍体乌鳢群体与三倍体群体的生长差异进行了比较研究。结果表明,(1)热休克法适宜诱导条件为,(28 ± 0.5)℃培育水温授精后4 min,在水温42 ℃条件下持续处理3 min,三倍体诱导率最高,达到87.69%;(2)三倍体与二倍体DNA含量差异极显著(P < 0.01),其比值为1.50︰1.00;(3)三倍体和二倍体在红细胞核长径、红细胞核体积、红细胞核面积等6项指标上存在极显著差异(P < 0.01);与二倍体相比,三倍体红细胞体积和核体积分别是其1.69倍和1.60倍;(4)在4月龄与8月龄,三倍体的体长和体重比二倍体稍高,但两者差异不显著(P > 0.05)。本实验结果为进一步开展乌鳢倍性育种奠定了基础。
英文摘要:
      Snakehead (Channa argus) is a commercially important freshwater fish cultured in China. The induction of triploidy can be used to control unwanted reproduction in culture and is an important breeding method in fish. Given this, the development of triploid stock would be of significant benefit for aquaculture. In the present study, the second polar body extrusion was inhibited by heat shock for triploidy induction in the Snakehead, and the optimal induction conditions were evaluated. Ploidy of putative triploid larvae was determined by means of DNA content and red blood cell size identification method. Meanwhile, the growth performance of triploids and diploids were compared. All the data were analyzed by using Microsoft excel 2003 and SPSS 13.0. The results showed that: (1) the highest triploid induction rate of 87.69% was observed when the fertilized eggs were shocked at 42℃ for 3min after fertilization 4 min, and incubated at water temperature of (28 ± 0.5)℃ (Table 3); (2) the cellular DNA content of triploid Snakehead was 1.50 times to that of the diploid (P < 0.01); (3) there were very significant differences in cell and nuclear sizes between the triploid and diploid erythrocytes (P < 0.01) (Fig. 2), and the erythrocyte volume and nucleus volume of triploids were 1.69 and 1.60 times of the diploids, respectively (Table 5); (4) from 4 to 8 months of age, the triploids growth rates (body length, weight) were not significantly different compared to the diploid controls (P > 0.05) (Table 6). In conclusion, this technique lays foundation and provides important tool for polyploid breeding in Snakehead.
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