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黄建盛,陈刚,张健东,汤保贵,王忠良,周晖.2016.温度、pH及体重对低盐水体下斜带石斑鱼幼鱼耗氧率与排氨率的影响.动物学杂志,51(6):1038-1048.
温度、pH及体重对低盐水体下斜带石斑鱼幼鱼耗氧率与排氨率的影响
Effects of Temperature, pH and Body Wet Weigh on Oxygen Consumption Rate and Ammonia Excretion Rate of Orange-spotted Grouper Epinephelus coioides Juveniles Cultured in Low-salt Water
投稿时间:2015-12-28  修订日期:2016-08-19
DOI:DOI: 10.13859/j.cjz.201606012
中文关键词:  斜带石斑鱼  耗氧率  排氨率  低盐水体
英文关键词:Orange-spotted Grouper, Epinephelus coioides  Oxygen consumption rate  Ammonia excretion rate  Low-salt water
基金项目:广东高校优秀青年创新人才培养计划项目(No. 2013LYM-0038),广东省海洋渔业科技推广专项项目[No. A2010-08D04,A2010004-016(c)],广东省海洋经济创新发展区域示范专项(No.GD2012-A02-003),广东海洋大学科研启动经费(No. E15183);
作者单位E-mail
黄建盛 广东海洋大学水产学院 fishaqu@126.com 
陈刚 广东海洋大学水产学院 cheng@ gdou.edu.cn 
张健东 广东海洋大学水产学院  
汤保贵 广东海洋大学水产学院  
王忠良 广东海洋大学水产学院  
周晖 广东海洋大学水产学院  
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中文摘要:
      在低盐水体(盐度12)条件下,利用实验生态学方法采用间歇式流水呼吸仪测定了不同温度(21℃、24℃、27℃、30℃及33℃)、pH(6.5、7.0、7.5、8.0及8.5)及体重(均值:15.64 g、35.80 g、65.67 g和95.93 g)对斜带石斑鱼(Epinephelus coioides)幼鱼耗氧率(MO2)和排氨率(MTAN)的影响。结果表明,温度对斜带石斑鱼幼鱼MO2和MTAN有显著性影响(P < 0.05),随着温度的上升均呈现上升的变化,二者与温度(T)之间的关系均可以用线性函数来拟合(MO2 = 6.0826T﹣8.9704,R2 = 0.9127;MTAN = 0.2248T﹣0.7731,R2 = 0.7792)。在实验温度范围,呼吸温度系数Q10和排泄温度系数Q10均值分别为1.51和1.54,而且在水温27℃和30℃时最小,这表明斜带石斑鱼适宜生长的温度范围为27 ~ 30℃。pH对斜带石斑鱼幼鱼MO2和MTAN有显著性影响(P < 0.05),随pH上升均呈现先升后降的变化,二者与pH之间的关系均可以用二次函数来拟合(MO2 =﹣15.241ApH2 + 234.98ApH﹣737.42,R2 = 0.7888;MTAN =﹣1.1477ApH2 + 18.073ApH﹣65.369,R2 = 0.7557)。体重对斜带石斑鱼幼鱼MO2和MTAN有显著性影响(P < 0.05),随着体重的增加均呈下降的变化,与体重(W)之间关系均可以用幂函数来拟合(MO2 = 310.61 W ﹣0.1972,R2 = 0.8653;MTAN = 9.9167W ﹣0.2043,R2 = 0.8257),而耗氧量(RO2)和排氨量(RTAN)随体重的增加均呈上升的变化,与体重之间的关系均可以用幂函数来拟合(RO2 = 0.3106W 0.8028,R2 = 0.9907;RTAN = 0.0099W 0.7957,R2 = 0.9863)。幼鱼的氧氮比均值在本实验温度范围为25.90,在本实验pH范围其为28.65,在本实验体重范围其为28.19。这提示斜带石斑鱼幼鱼在低盐水体养殖主要以蛋白质和脂肪为能量来源。
英文摘要:
      Orange-spotted Grouper (Epinephelus coioides) which inhabits subtropical and tropical areas, has become a very popular species of marine teleost currently being cultured in China. Metabolic rate is the most fundamental biological rate as it represents the rate of energy uptake, transformation and allocation. Oxygen consumption is a widely studied indicator of metabolic rate, and measurement of oxygen consumption rate is often used to examine energy utilization and stress in aquatic animals. The aim of the present study is to investigate the effects of temperature (21℃, 24℃, 27℃, 30℃ and 33℃), pH (6.5, 7.0, 7.5, 8.0 and 8.5) and body wet weight (mean values:15.64 g, 35.80 g, 65.67 g and 95.93 g) on oxygen consumption rate (MO2), ammonia excretion rate (MTAN) of Orange-spotted Grouper juveniles cultured in low-salt water (salinity 12) by the intermittent flow respirometry system with ecological methods in laboratory. Data were analyzed by using the one-way ANOVA, followed by Ducan test. All analyses were performed with a significance level of P < 0.05. The results showed that temperature strongly affected both MO2 and MTAN. The MO2 and MTAN increased significantly (P < 0.05) with an increase in temperature from 21℃ to 33℃ (Table 1). The linear equation between the MO2, MTAN and temperature (T) were established (MO2 = 6.0826T﹣8.9704, R2 = 0.9127; MTAN = 0.2248T﹣0.7731, R2 = 0.7792) (Fig. 2). Over the entire experimental temperature range (21﹣33℃), the respiration temperature coefficient Q10 and excretion temperature coefficient Q10 were 1.51, 1.54, respectively, and the lowest values were found between 27 and 30℃. The optimal temperature for the juvenile lied between 27 and 30℃ (Table 2). Both MO2 and MTAN were significantly affected by pH (P < 0.05) (Table 1), being increased first then decreased with pH increase. The relationship between MO2, MTAN and pH was modeled by a quadratic equation (MO2 = ﹣15.241ApH2 + 234.98ApH ﹣737.42, R2 = 0.7888; MTAN =﹣1.1477ApH2 + 18.073ApH﹣65.369, R2 = 0.7557) (Fig. 2). Both MO2 and MTAN decreased significantly with increased body wet weight (P < 0.05) (Table 1), and their relationship with body wet weight (W) could be represented by power equations (MO2 = 310.61W ﹣0.1972, R2 = 0.8653; MTAN = 9.9167W ﹣0.2043, R2 = 0.8257). The oxygen consumption (RO2) and ammonia excretion (RTAN) increased with increase in body mass, and their relationship with body wet weight was modeled by power equations (RO2 = 0.3106W 0.8028, R2 = 0.9907; RTAN = 0.0099W 0.7957, R2 = 0.9863). The averages of oxygen-nitrogen ratio (O︰N) at different temperatures, pH values and body wet weights were 25.90, 28.65 and 28.19, respectively (Table 1), which indicated that Orange-spotted Grouper juvenile cultured in low- salt water utilized protein-lipid dominated metabolism.
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