Six specimens of odour frogs were collected from Fanjing Mountain National Nature Reserve (FMNNR) in Guizhou Province from May to July in 2015 (Table 1). The specimens were identified as Odorrana yizhangensis (Amphibia, Anura, Ranidae); and this is the new record of this species in Guizhou Province. Twenty two morphological characters of the specimens were measured (Table 2) and their habitats (Fig. 1) were also described. The maximum likelihood phylogenetic tree constructed based on 12S rRNA and 16S rRNA gene fragments showed that the six specimens were clustered with the O. yizhangensis sampled from its type locality (Fig. 2), and the genetic distance of Kamura-2-parameter model between our specimens and O. yizhangensis sample from its type locality was much smaller than that between Odorrana species (Table 3). New found of O. yizhangensis in Guizhou Province would promote taxonomic and phylogeographic studies on this species.