| 王蕊,苟锐锋,王树林.2016.营养丰盛期和营养匮乏期牦牛组织中BCMO1蛋白表达.动物学杂志,51(6):1003-1009. |
| 营养丰盛期和营养匮乏期牦牛组织中BCMO1蛋白表达 |
| Expression of BCMO1 Protein in Yak (Bos mutus) Tissues during Different Nutrition Periods |
| 投稿时间：2015-12-08 修订日期：2016-08-18 |
| DOI：DOI: 10.13859/j.cjz.201606008 |
| 中文关键词: β胡萝卜素-15, 5′-单加氧酶 蛋白质免疫印迹技术 营养平衡 |
| 英文关键词:β-carotene-15, 15′-monooxygenase 1 Western blotting Nutrition balance |
| 基金项目:青海省高原放牧家畜营养与生态省部共建国家重点实验室培育基地开放课题，科技部973专项（No. 2012CB722906）； |
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| 全文下载次数: 2013 |
| 中文摘要: |
| 通过研究营养匮乏期和丰盛期牦牛（Bos mutus）体内β胡萝卜素-15, 5′-单加氧酶（BCMO1）蛋白表达水平，探讨不同营养时期BCMO1参与牦牛机体维持维生素A营养平衡的功能。于2013年7月（营养丰盛期）和2014年3月（营养匮乏期）赴青海省海西州乌兰县铜普镇采集样品，每次选取健康成年（36月龄）雄性牦牛3头，采集组织样品。采用蛋白质免疫印迹技术检测不同营养时期牦牛肝、肺、肾、肌肉、十二指肠、回肠、空肠、瘤胃8种组织中BCMO1蛋白的表达量。利用多重比较和T检验对同一时期不同组织及不同时期同一组织BCMO1蛋白表达量进行比较分析。在营养匮乏期，BCMO1蛋白表达量最高的组织为十二指肠（P < 0.05）；空肠和回肠组织中BCMO1蛋白表达量高于肾和瘤胃（P < 0.05）；肝组织中BCMO1蛋白表达量最低。在营养丰盛期牦牛肝组织中BCMO1蛋白表达量最高，明显高于其他组织（P < 0.05）。营养丰盛期肝中的BCMO1蛋白表达量为0.919 ± 0.228，显著高于营养匮乏期（P < 0.05）；而营养丰盛期牦牛十二指肠、空肠、肾、肌肉和瘤胃组织中的BCMO1蛋白表达量分别为0.811 ± 0.134、0.336 ± 0.255、0.739 ± 0.404、0.619 ± 0.092，均低于营养匮乏期表达量（P < 0.05）；在营养丰盛期和营养匮乏期的肺中BCMO1蛋白表达量分别为1.128 ± 0.407、1.083 ± 0.232，回肠在营养丰盛期和营养匮乏期的BCMO1蛋白表达量分别为0.466 ± 0.337、0.517 ± 0.280，两组织在不同时期BCMO1蛋白表达量差异不明显（P > 0.05）。研究表明，在牦牛体内的β-胡萝卜素和维生素A含量较高的营养丰盛期，大部分牦牛组织中BCMO1蛋白表达量较匮乏期要低，体内维生素A的含量提高可能对BCMO1的表达有反馈抑制作用，这对维持和调节牦牛在不同营养时期机体内维生素A营养平衡具有重要意义。 |
| 英文摘要: |
| In order to explore the function of β-carotene-15, 15′-monooxygenase 1 (BCMO1) in vitamin A (VA) metabolic balance in Yak (Bos mutus), the expression levels of BCMO1 protein were studied in Yak tissues at different nutrition periods. Three healthy adult males of 3 years old were selected for samples collection at July 2013 (nutrition-rich period) and March 2014 (nutrition-deficiency period) at Tongpu town of Wulan County in Qinghai Province. Western blotting were used to detect the expression of BCMO1 protein in eight tissues including liver, lung, kidney, muscle, duodenum, ileum, jejunum and rumen. The different expression levels of BCMO1 protein in different tissues at the same nutrition stage was compared using multiple comparisons and the difference of the expression level at different nutrition periods for the same tissue was compared using T test. As shown in Fig. 1 and Fig. 2, there was the highest expression level of BCMO1 protein in duodenum among all tissues (P < 0.05), and the expression level in ileum and jejunum tissue was higher than that of kidney and rumen (P < 0.05). BCMO1 protein expression level was the lowest in liver among all tissues during the nutrition-deficiency period. During the nutrient-rich period, liver tissue showed the highest level of BCMO1 protein among all tissues (P < 0.05). As shown in Fig. 2 and Table 1, the expression of BCMO1 protein in the liver was 0.919 ± 0.228 at nutrition-rich period, which was significantly higher than that at nutrient-deficiency period (P < 0.05). However, the expression levels of BCMO1 protein in duodenum, jejunum, kidney, muscle and rumen were 0.811 ± 0.134, 0.336 ± 0.255, 0.739 ± 0.404, 0.619 ± 0.092 respectively at nutrition-rich period, which were lower than those at nutrition-deficiency period (P < 0.05). The BCMO1 expression levels in lung were 1.128 ± 0.407 and 1.083 ± 0.232, and its expression levels in ileum were 0.466 ± 0.337 and 0.517 ± 0.280 at nutrition-rich and nutrition-deficiency periods, respectively. There was no significant difference between two different nutritional periods for BCMO1 expression in two tissues (P > 0.05). The expression of BCMO1 protein was lower at nutrition-rich period than that at the nutrition-deficiency period for most tissues, which indicated that sufficient content of vitamin A in tissues might inhibit expression of BCMO1, as the contents of β-carotene and vitamin A were adequate in Yak tissues at nutrition-rich time. It is very important to maintain and regulate vitamin A metabolic balance in Yak at different nutrition periods. |
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