| 杨燕燕,俞春英,周建华,王训立.2016.FMR1基因敲除对C57BL/6小鼠部分生物学特性的影响.动物学杂志,51(6):1010-1017. |
| FMR1基因敲除对C57BL/6小鼠部分生物学特性的影响 |
| The Influence of FMR1 Gene Knockout on Some Biological Characteristics of C57BL/6 Mice |
| 投稿时间：2015-12-07 修订日期：2016-08-25 |
| DOI：DOI: 10.13859/j.cjz.201606009 |
| 中文关键词: 基因敲除 脆性X智力障碍1（FMR1）基因 C57BL/6小鼠 血液理化指标 |
| 英文关键词:Gene knockout Fragile X retardation 1 gene (FMR1) C57BL/6 mice Physiological and biochemical indexes |
| 基金项目:福建省实验动物研究重点项目（No. 2014Y0079）； |
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| 中文摘要: |
| 为探讨脆性X智力障碍1（FMR1）基因敲除对C57BL/6小鼠（Mus musculus domesticus）部分生物学特性的影响，使用全自动动物血细胞分析仪和全自动生化仪分别检测8 ~ 10周龄的FMR1基因敲除小鼠（C57BL/6 FMR1 KO）及同源背景C57BL/6小鼠的血液生理生化指标、电解质等，并进行统计学分析。C57BL/6 FMR1 KO小鼠与野生型C57BL/6小鼠比较，血液生理指标中雌性及雄性组间的中性粒细胞计数（MEUT#）、中性粒细胞百分比（MEUT）和淋巴细胞百分比（LY）存在显著性差异（P < 0.05）；雄性组间的红细胞总数（RBC）、血红蛋白（HGB）、红细胞压积（HCT）和平均血红蛋白浓度（MCHC）存在显著性差异（P < 0.05）；雌性组间的白细胞（WBC）、淋巴细胞计数（LY#）存在显著性差异（P < 0.05）；而非性别因素影响两类小鼠组间的红细胞总数（RBC）、红细胞压积（HCT）、中性粒细胞计数（MEUT#）、中性粒细胞百分比（MEUT）和淋巴细胞百分比（LY）存在显著性差异（P < 0.05）；血清生化指标中雄性组间的谷草转氨酶/谷丙转氨酶（AS/AL）存在显著性差异（P < 0.05）；雌性组间的肌酐CR、肌酸磷酸激酶CK和钙离子浓度Ca2+存在显著性差异（P < 0.05）；而非性别因素影响两类小鼠组间的谷草转氨酶/谷丙转氨酶（AS/AL）、肌酐CR和肌酸磷酸激酶CK存在显著性差异（P < 0.05）；其他各项指标差异不显著（P > 0.05）。研究表明，FMR1基因敲除可影响小鼠的部分生理生化指标水平，这为今后研究和应用C57BL/6 FMR1 KO小鼠模型提供了实验依据。 |
| 英文摘要: |
| This study aims to explore the influence of fragile X retardation 1 (FMR1) gene knockout on biological characteristics of C57BL/6 (Mus musculus domesticus) mice. Blood physiological and biochemical indexes of FMR1 knockout mice and C57BL/6 mice of 8﹣10 weeks old were respectively examined using animal automatic blood cell analyzer and automatic biochemical detection. The comparison of difference between groups was analyzed with independent-samples T test method using SPSS 19.0. The blood routine indexes of C57BL/6 FMR1 KO male and female mice, such as neutrophilic granulocyte (MEUT#), neutrophilic granulocyte percentage (MEUT) and lymphocyte percentage (LY), had significant difference compared to C57BL/6 mice (P < 0.05); for males, the number of red blood cells (RBC), hamoglobin (HGB), hematocrit value (HCT) and mean corpuscular hemoglobin concentration (MCHC) showed significant difference compared to C57BL/6 mice (P < 0.05); for females, the number of white blood cells (WBC) and lymphocytes (LY#) showed significant difference compared to C57BL/6 mice (P < 0.05); the non-sex factors including red blood cells (RBC), hematocrit value (HCT), neutrophilic granulocytes (MEUT#), neutrophilic granulocyte percentage (MEUT) and lymphocyte percentage (LY) showed significant difference compared to C57BL/6 mice (P < 0.05). But there was no significant difference in the number of platelets (PLT), mean platelet volume (MPV), plateletcrit (PCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), red blood cell distribution width-standard deviation (RDW-SD), red blood cell distribution width-coefficient of variance (RDW-CV), platelet distribution width (PDW), platelet-large cell rate (P-LCR), monocytes (MONO #), monocytes percentage (MONO), eosnophils (EO#), eosnophils percentage (EO), basophilic granulocytes (BASO#) and basophilic granulocyte percentage (BASO) between C57BL/6 FMR1 KO mice and C57BL/6 mice (P > 0.05) (Table 1). Serum biochemical indexes of C57BL/6 FMR1 KO mice, such as the AST/ALT (AS/AL) in males had significant difference compared to C57BL/6 mice (P < 0.05); the creatinine (CR), creatine phosphokinase (CK) and electrolyte Ca2+ in females had significant difference compared to C57BL/6 mice (P < 0.05); the non-sex factors including AST/ALT (AS/AL), creatinine (CR) and creatine phosphokinase (CK) had significant difference compared to C57BL/6 mice (P < 0.05). No significant difference was found in albumin (ALB), globulin (GLB), ALB/GLB (A/G), alkaline phosphatase (ALP), glutamyltransferase (GGT), total protein (TPROT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), uric acid (UA), cholesterol (CHO), triglyceride (TG), glucose (GLU), lactic dehydrogenase (LDH), osmotic pressure, K+, Na+, Cl￣, Mg2+ and P3+ (P > 0.05) (Table 2). Thus, the results suggest that FMR1 gene knockout can influence some blood physiological and serum biochemical values in mice. These results will provide the experimental basis for researching and using C57BL/6 FMR1 KO mouse model in the future. |
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