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陈卓,罗静,张国钢,何宏轩.2016.青海湖斑头雁粪便细菌分离鉴定与耐药性分析.动物学杂志,51(4):590-598.
青海湖斑头雁粪便细菌分离鉴定与耐药性分析
Isolation, Identification and Antimicrobial Susceptibility of Fecal Bacteria from Bar-headed Geese Anser indicus from Qinghai Lake
投稿时间:2015-11-19  修订日期:2016-03-15
DOI:DOI: 10.13859/j.cjz.201604010
中文关键词:  斑头雁  粪便细菌  分离鉴定  耐药性
英文关键词:Anser indicus  Fecal bacteria  Isolation and identification  Antibiotic resistance
基金项目:国家林业局野生动物疫源疫病项目专项;中国科学院战略生物资源科技支撑体系运行专项(CZBZX-1);“十二五”国家科技支撑计划:野生动物跨境传播重要疫病监测与防控技术研究(2013BAD12B04)
作者单位E-mail
陈卓 中国林业科学研究院森林生态环境与保护研究所
中国科学院动物研究所 
1053526368@qq.com 
罗静 中国科学院动物研究所  
张国钢 中国林业科学研究院森林生态环境与保护研究所  
何宏轩 中国科学院动物研究所 hehx@ioz.ac.cn 
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中文摘要:
      为了解斑头雁粪便中携带细菌多样性及其耐药情况,对青海湖斑头雁粪便细菌进行分离培养和生化鉴定、16S rRNA基因PCR扩增、序列分析,并进行细菌耐药性试验。结果显示,从30份斑头雁粪便中共分离到123株、10类细菌,主要有大肠埃希菌、水生拉恩氏菌、蒙氏肠球菌、枯草芽孢杆菌、柠檬节杆菌、腐败希瓦氏菌、河生肠杆菌、成团泛菌、杀鲑气单胞菌和产酸克雷伯菌。其中水生拉恩菌、杀鲑气单胞菌和成团泛菌表现为多重耐药性;其他细菌对氨苄西林和四环素有一定的耐药性,对其余受试药物都有不同程度的敏感性。表明斑头雁粪便中携带一些条件致病菌,并存在一定的耐药性,这对对野生动物疫病的监测与防控有重要意义,也为研究野鸟携带细菌的耐药机制提供理论依据。
英文摘要:
      Fecal bacteria from Anser indicus in Qinghai Lake were isolated and identified by colonial morphology observation, biochemical properties tests, amplification of 16S rRNA genes by PCR and sequences analysis. The resistance of isolates to 13 drugs were evaluated either. The results shows that 123 bacteria strains which belongs to 10 genus were isolated from 30 feces, they were Escherichia coli,Rahnella aquatilis, Enterococcus mundtii, Bacillus sublitis, Arthrobacter citreus, Shewanella pulrefaciens, Enterobacter amnigenus, Pantoea agglomerans, Aeromonas salmonicida, Klebsiella oxytoca respectively. Among these bacteria, Rahnella aquatilis, Aeromonas salmonicida and Pantoea agglomerans were multidrug resistant, and other bacteria were resistant to Ampicillin and tetracycline. The results obtained from this study indicated that Anser indicus would take amounts of pathogen which exhibit antibiotic resistance. It is of great value to the surveillance of wildlife disease, and provides references to study the antibiotic resistance mechanicsm of bacteria in wild birds.
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