| 姜永华,王艺磊,张子平,洪万树.2016.RNAs介导的干细胞诱导技术研究进展.动物学杂志,51(4):692-706. |
| RNAs介导的干细胞诱导技术研究进展 |
| RNAs-mediated Technologies for Inducing Pluripotent Stem Cells |
| 投稿时间：2015-11-18 修订日期：2016-05-13 |
| DOI：DOI: 10.13859/j.cjz.201604023 |
| 中文关键词: 诱导性多能干细胞 RNAs 研究进展 |
| 英文关键词:Induced pluripotent stem cells RNAs Research progress |
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| 中文摘要: |
| 诱导性多能干细胞（iPSCs）技术可重编程体细胞为胚胎干细胞（ESCs）样的多能性细胞，在药物筛选、再生医学等领域具有巨大的应用潜力。iPSCs技术自2006年首次报道用逆转录病毒转导一组转录因子，将小鼠（Mus musculus）成纤维细胞成功重编程为iPSCs以来，便不断改进和完善。近年来，不引起任何基因组改变的RNAs介导的iPSCs技术成为新兴的研究热点，主要包括修饰mRNAs法、miRNAs法、siRNAs法和lncRNAs法等。本文综述了RNAs介导的各种iPSCs技术的研究进展，分析了这些技术的优势、存在的不足及改进的方向等，为iPSCs技术的发展与应用提供参考。 |
| 英文摘要: |
| Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs), which may replace embryonic stem cells (ESCs) and have great application potential in drug screening, regenerative medicine, etc. Since the iPSCs development by retroviral transduction of a defined set of transcription factors into fibroblasts (mF) was first reported in 2006 in mouse (Mus musculus), more new strategies have been developed to improve and refine the reprogramming technology. Recent technology of RNAs-mediated iPSCs becomes an emerging research hotspot which provides an optimism for the generation of safe iPSCs without any genomic modification. RNAs-mediated iPSCs technology includes the application of modified mRNAs, miRNAs, siRNAs and lncRNAs. In this paper, the research progress of various RNAs-mediated iPSCs technologies are reviewed, including the advantages, deficiencies and the improvement of these techniques, so as to provide references for the development and application of iPSCs technology. |
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