| 赵丹阳,江峰,孙拂晓,周永康,王朝林,吴孝兵.2016.超声成像在扬子鳄卵巢等器官形态检测中的应用.动物学杂志,51(5):914-921. |
| 超声成像在扬子鳄卵巢等器官形态检测中的应用 |
| Ultrasonography of Ovaries and Other Organs in Chinese Alligator (Alligator sinensis) |
| 投稿时间：2015-09-09 修订日期：2016-08-26 |
| DOI：DOI: 10.13859/j.cjz.201605022 |
| 中文关键词: 扬子鳄 卵巢 卵泡 超声成像（B超） |
| 英文关键词:Chinese Alligator, Alligator sinensis Ovary Follicles Ultrasonography |
| 基金项目:国家自然科学基金项目（No. 31472019，31272337） |
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| 中文摘要: |
| 扬子鳄（Alligator sinensis）是中国特有的珍稀物种，该物种的生长、繁殖状况一直备受关注，分别在2014年3月（冬眠期）和6月（繁殖期）对17条成年雌性扬子鳄腹部应用VIVIDⅠ型彩色多普勒超声（B型超声）便携诊断仪进行检测，并对3月份卵巢卵泡大小用SPSS 19.0回归分析。扬子鳄心、肝、肠等内脏器官形态在B型超声诊断仪下清晰可见。冬眠期17条扬子鳄两侧卵巢中共检测到41枚卵泡，繁殖期共42枚。冬眠期卵泡处于低回声暗区或无回声暗区的未成熟状态，繁殖期多数卵泡的发育状态与3月相同，少数卵泡呈高回声暗区，此时已形成卵黄颗粒，也有呈高回声亮光区形成卵黄膜的卵泡。对3月份检测的17条扬子鳄具有卵泡的19个卵巢大小与卵泡大小进行回归分析，卵巢随卵泡发育而增大。本研究探讨了B超这种低损伤方法对扬子鳄检测的有效性，同时评估卵泡发育状况，提高繁殖鳄的筛选正确率。 |
| 英文摘要: |
| Chinese Alligator (Alligator sinensis) is a rare species endemic to China. Up to now, the growth and reproduction of Chinese Alligator have been received much attention. In this paper, we used the color doppler ultrasonic diagnostic apparatus (VIVIDⅠ) to examine the abdomen of 17 adult female alligators in March (hibernating period) and June (breeding season) 2014, respectively, and the sizes of ovarian follicles in March was analyzed by regression analysis using SPSS 19.0 software. The internal organs including heart, liver and intestine were clearly observed. A total of 41 follicles during hibernating period and 42 follicles during breeding season were examined. Follicles in low or no echo dark area were immature during hibernation. Follicular development in reproduction period was basically the same as in March. A handful of follicles in high echo dark space had formed yolk granules while others in high echo light area had formed the vitelline membrane. Regression analysis of 19 follicles′sizes indicated that the ovary size increased with the follicular development. The shapes of organs as well as the structure of heart were readily discernible with ultrasonography. Process of blood flow in heart could be detected by color doppler ultrasound. Heart rates were 12 and 43 counts per minute in hibernating period and breeding season, respectively. We show that ultrasonography is a low-invasive way that can be used to assess follicular development. In Chinese Alligator, which increases accuracy of sieving in breeding alligator. |
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