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王悦,杨丽珠,方静,彭西,樊均德.2016.β-内啡肽在齐口裂腹鱼消化道和脑中的定位分布.动物学杂志,51(3):404-412.
β-内啡肽在齐口裂腹鱼消化道和脑中的定位分布
The Distribution Patterns of β-endorphin in the Digestive Tract and Brain of Schizothorax prenanti
投稿时间:2015-09-03  修订日期:2016-05-10
DOI:DOI: 10.13859/j.cjz.201603008
中文关键词:  β-内啡肽  齐口裂腹鱼  消化道    免疫组织化学
英文关键词:β-endorphin  Schizothorax prenanti  Digestive tract  Brain  Immunocytochemical method
基金项目:教育部长江学者和创新团队发展计划(0848),贵州省教育厅工程中心建设项目(黔教合KY[2012]028号),贵州省教育厅高等学校重点支持学科(黔教合重点支持学科字[2011]232号)
作者单位E-mail
王悦 四川农业大学动物科技学院 wangyue20120642@163.com 
杨丽珠 四川农业大学动物医学院  
方静 四川农业大学动物医学院 fangjing4109@163.com 
彭西 四川农业大学动物医学院  
樊均德 铜仁学院生物与农林工程学院  
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中文摘要:
      β-内啡肽属内源性阿片肽,广泛存在于胃肠道及脑组织中,发挥着多种生理功能。本文采用链霉亲和素-生物素复合物免疫组织化学技术研究β-内啡肽在齐口裂腹鱼(Schizothorax prenanti)消化道和脑中的定位分布。食道、后肠呈β-内啡肽阴性反应,口咽腔、前肠及中肠呈β-内啡肽阳性反应,阳性细胞多分布于黏膜上皮之间,呈圆形、卵圆形、蝌蚪形、梭形、锥形等。β-内啡肽阳性细胞分布密度在前肠最高,中肠次之,口咽腔最低。β-内啡肽定位于间脑、中脑、小脑神经元及中脑神经纤维中,其阳性神经元密度在中隆起最高,下丘脑中叶前球核、下丘脑下叶次之,侧膝核、前圆核、下圆核、中纵束旁及小脑瓣浦肯野氏细胞层较低,下丘脑下叶乳头体、中脑基部上缘最低。β-内啡肽在消化道的分布与该鱼食性及消化道结构、功能密切相关;β-内啡肽在脑区的分布特性,推测与其参与调节不同脑区的神经内分泌活动有关。综上所述,β-内啡肽广泛分布于齐口裂腹鱼消化道及脑内,进一步证实它是一种双重分布的脑肠肽。本研究为β-内啡肽可能参与调节消化、神经内分泌活动提供了形态学证据。
英文摘要:
      β-endorphin is a kind of brain-gut peptide and plays important physiological roles. The distributive patterns of β-endorphin in the digestive tract and brain of Schizothorax prenanti were studied using strept avidin-biotin complex (SABC) immunocytochemical method. β-endorphin-positive cells were observed through Olympus microscope and the number and optical density of β-endorphin-positive cells were measured using image analysis software and expressed as Mean ± standard deviations. The results showed that the positive immunoreactions presented brown yellow color (Plate I: 1-14), while negative control in the foregut showed only hematoxylin staining (Plate I: 15). There was no β-endorphin-positive reaction in the esophagus or hindgut. β-endorphin-positive cells were detected in the oropharyngeal cavity (Plate I: 1), foregut (Plate I: 2) and midgut (Plate I: 3), presenting various kinds of forms, such as round, ovoid, tadpole, spindle and cone shapes. The density of β-endorphin-positive cells in the digestive tract was the highest in the foregut (13.10 ± 4.50 number/mm2), medium in the midgut (10.08 ± 2.96 number/mm2) and the lowest in the oropharyngeal cavity (8.52 ± 3.72 number/mm2) (Table 1). β-endorphin-positive reaction was located in the neurons of diencephalon (Plate I: 4-9), mesencephalon (Plate I: 11-13) and metencephalon (Plate I: 14), and the nerve fibers of mesencephalon (Plate I: 10). The density of β-endorphin-positive neurons in the brain was the highest in the eminentia medialis (248.42 ± 84.25 number/mm2), medium in the nucleus anterior tuberis of medium lobe of hypothalamus (150.42 ± 15.08 number/mm2) and inferiors lobes of hypothalamus (141.80 ± 45.36 number/mm2), lower in the nucleus geniculatus lateralis (98.44 ± 28.34 number/mm2), nucleus prerotundus (86.21 ± 18.45 number/mm2), nucleus subrotundus (92.81 ± 11.19 number/mm2), side of fasciculus longitudinalis medialis (95.10 ± 8.23 number/mm2) and layer of purkinje cells of cerebella valvula (88.73 ± 41.20 number/mm2), and the lowest in the corpus mammillare of inferiors lobes of hypothalamus (60.72 ± 10.39 number/mm2) and upper area of tegmentum (47.27 ± 11.95 number/mm2) (Table 1). The distribution patterns of β-endorphin-positive cells in the digestive tract of Sehizothorax prenanti are greatly related to its feeding habits, intestinal functions and structures as well. The distributive characteristics of β-endorphin-positive cells in the brain may be relevant to neuroendocrine activities in different areas of the brain. β-endorphin was widely distributed in the digestive tract and brain of Schizothorax prenanti, further suggesting that this molecular is a kind of brain-gut peptide. This study provided morphological evidence for regulating digestive and neuroendocrine activities by β-endorphin.
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