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王丽敏,杨纯,郭俐.2016.秦岭滑蜥消化系统组织结构及消化管嗜银细胞观察.动物学杂志,51(4):614-622.
秦岭滑蜥消化系统组织结构及消化管嗜银细胞观察
Histology of Digestive System and Argyrophil Cells in Digestive Tube of Tsinling Dwarf Skink (Scincella tsinlingensis)
投稿时间:2015-08-07  修订日期:2016-07-04
DOI:DOI: 10.13859/j.cjz.201604013
中文关键词:  秦岭滑蜥  消化系统  组织学  嗜银细胞
英文关键词:Scincella tsinlingensis  Digestive system  Histomorphology  Argyrophil cells
基金项目:国家级大学生创新创业训练计划项目(No. 201510118001)
作者单位E-mail
王丽敏 山西师范大学生命科学学院 临汾 041004 18203472865@163.com 
杨纯 山西师范大学生命科学学院 临汾 041004 yangchun774@163.com 
郭俐 山西师范大学生命科学学院 临汾 041004  
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中文摘要:
      为揭示秦岭滑蜥(Scincella tsinlingensis)消化系统的基本特征,运用大体解剖与组织切片技术、Grimelius浸银法对其消化系统组织结构以及消化管嗜银细胞的分布、形态和密度进行了观察。除舌外,消化管的管壁分为黏膜层、黏膜下层、肌层和外膜。消化管各部分的差异主要在管壁厚度和黏膜皱襞数量。各段管壁厚度以胃幽门部最厚,达(221.03 ± 5.94)μm,而十二指肠最薄,仅(63.59 ± 1.17)μm。各段黏膜皱襞的形态和数量明显不同,空肠多达17 ~ 20个,其次为回肠和十二指肠,分别为15 ~ 17个和11 ~ 13个。此外,消化管各部分肌层的相对厚度及腺体的分布也存在差异。肝组织无典型的肝小叶分化,胰腺中胰岛不发达。在秦岭滑蜥的消化管中,锥形、椭圆形、圆形或不规则形的嗜银细胞广泛分布于固有膜和黏膜上皮细胞之间,胃体部的密度最高。上述结果表明,秦岭滑蜥消化系统结构与大多数爬行类相比,无明显差异。消化管中嗜银细胞分布、形态和密度可能与其在该物种中的功能有关。
英文摘要:
      To reveal the basic characteristics of the digestive system of Tsinling dwarf skink (Scincella tsinlingensis), gross anatomy, paraffin section, H.E staining and Grimelius silver staining were used to observe the histomorphology of digestive system, the distribution density and the morphology of argyrophil cells in digestive tube. Statistical analysis is performed using Duncan multiple comparison test (SPSS 17.0 software). The H.E staining shows that in addition to the tongue, the tissue structure of digestive tube can be divided into mucosa, submucosa, muscularis and adventitia. The tube’s thickness of the pyloric part of stomach is the thickest (221.03 ± 5.94 μm) (Fig. 2e) and the duodenum is the thinnest (63.59 ± 1.17 μm) (Fig. 2f). The number of mucosal fold is different among digestive tubes. There are 17 - 20 folds in jejunum (Fig. 2h), followed by ileum (15 - 17) (Fig. 2i) and duodenum (11 - 13) (Fig. 2f). The thickness of muscularis is also different in different part of the digestive tube, and the pyloric part of stomach is the thickest (145.19 ± 3.27 μm) (Fig. 2e). There are many gastric glands lies in the lamina propria of the gastric mucos, such as cardiac gland, fundic gland and pyloric gland (Fig. 2c-e) and some intestinal glans in the lamina propria of the duodenum (Fig. 2f). Moreover, the epithelium mucosa of duodenum contains a little amount of goblet cells (Fig. 2g). The hepar parenchyma is composed of liver cells and the boundaries among hepatic lobule are not distinct. Some blood sinus, pigment cells, interlobular veins and interlobular bile ducts can be observed in the hepar’s connective tissue (Fig. 3a, b). The islets of pancreas are poorly developed (Fig. 3c). The Grimelius silver staining showes that argyrophil cells were widely distributed in the digestive tube except pharynx (Fig. 4a). The argyrophil cells are mainly distributed in the lamina propria, mucosal epithelium and glandular epithelium (Fig. 4). The shapes of argyrophil cells display cone-shape, oval, round or other irregular shapes (Fig. 4). The density of argyrophil cells in the body of stomach is the highest (16.80 ± 1.24), and the jejunum is the lowest (1.40 ± 0.24). There is extremely significant difference between the two parts (P<0.01) (Table. 1). Compared with most reptiles, the digestive system of the Tsinling dwarf skink shows little structural difference. The distribution and morphology of argyrophil cells in the digestive tube are likely consistent with their function in S. tsinlingensis.
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