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王鑫伟,史应学,魏平,竺俊全,吴雄飞.2016.乙二醇降低超低温冷冻黄姑鱼精子的DNA损伤.动物学杂志,51(2):261-267.
乙二醇降低超低温冷冻黄姑鱼精子的DNA损伤
The DNA Damage of Cryopreserved Sperm Is Declined by Using Ethylene Glycol as a Cryoprotectant in Spotted Maigre (Nibea albiflora)
投稿时间:2015-07-22  修订日期:2016-02-15
DOI:DOI: 10.13859/j.cjz.201602012
中文关键词:  黄姑鱼  精子  超低温冷冻保存  活力  DNA损伤
英文关键词:Nibea albiflora  Spermatozoa  Cryopreservation  Motility  DNA damage
基金项目:浙江省水产新品种选育专项(No.2012C12907-8),宁波市科技计划重大项目(2015C110005),国家海洋局海洋药源生物种质资源库建设项目(12PYY001SF08-NBDX-1),浙江海洋高效健康养殖协同创新中心项目;
作者单位E-mail
王鑫伟 宁波大学教育部应用海洋生物技术重点实验室 浙江宁波 315211② 宁波市海洋与渔业研究院 浙江宁波 315012 wxw199606@163.com 
史应学 宁波大学教育部应用海洋生物技术重点实验室 浙江宁波 315211② 宁波市海洋与渔业研究院 浙江宁波 315012  
魏平 宁波大学教育部应用海洋生物技术重点实验室 浙江宁波 315211② 宁波市海洋与渔业研究院 浙江宁波 315012  
竺俊全 宁波大学教育部应用海洋生物技术重点实验室 浙江宁波 315211② 宁波市海洋与渔业研究院 浙江宁波 315012 zhujunquan@nbu.edu.cn 
吴雄飞 宁波大学教育部应用海洋生物技术重点实验室 浙江宁波 315211② 宁波市海洋与渔业研究院 浙江宁波 315012  
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中文摘要:
      以HBSS溶液为稀释液,EG为抗冻剂,0.5mL麦细管为冻存管,两步降温法超低温冷冻保存黄姑鱼精子,并用单细胞凝胶电泳技术(SCGE)检测了冻融精子的DNA损伤。结果表明:5%、10%、15%EG组冻精的运动时间及寿命与鲜精相比差异不显著;20%、25%、30%EG组冻精的运动时间及寿命与鲜精相比差异显著。5%、10%、15%、20%EG组冻精核DNA损伤状况与鲜精无显著差异,25%、30%EG组冻精核DNA损伤状况与鲜精差异显著,且冻精核DNA的损伤程度与EG浓度成正相关。5%~15%EG适宜作为黄姑鱼精子超低温冷冻保存用抗冻剂。
英文摘要:
      In this paper, we studied the spermatozoa cryopreservation of Nibea albiflora using two-step cooling methods, HBSS as extender, EG(ethylene glycol) as cryoprotectant, in 0.5mL straws, also investigated the DNA damage in response to the cryopreservation process by SCGE. The results showed that there were no significant differences between fresh sperm and frozen-thawed sperm which diluted with 5%, 10% or 15%EG in moving time and life-span time. However, frozen-thawed sperm moving time and life-span time with 20%, 25% or 30% EG as cryoprotectant had a significant drop as compared with fresh sperm. The SCGE results demonstrated that there were no significant differences between fresh sperm and frozen-thawed sperm which diluted with 5%, 10%, 15% or 20%EG in DNA fragments, but DNA fragments differed significantly with fresh sperm when EG concentrations was added to 25% or 30%. In fact, there was a positive correlation between DNA damage extent of frozen-thawed sperm and concentrations of EG in protocol. So we conclude that cryoprotectant of 5%~15%EG can be considered as the most effective choices for spermatozoa cryopreservation of Nibea albiflora.
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