| 杨莎莎,徐志文,王承东,李才武,朱玲,周远成,李新琼,温安祥,李德生.2017.重组大熊猫IFN-γ聚氰基丙烯酸正丁酯纳米球的制备与评价.动物学杂志,52(2):314-321. |
| 重组大熊猫IFN-γ聚氰基丙烯酸正丁酯纳米球的制备与评价 |
| Preparation and Evaluation of Recombinant Interferon-gamma Nanosphere Loaded in Polybutylcyanoacrylate in Giant Panda (Ailuropoda melanoleuca) |
| 投稿时间：2015-07-11 修订日期：2016-12-08 |
| DOI：10.13859/j.cjz.201702015 |
| 中文关键词: 大熊猫 干扰素γ 聚氰基丙烯酸正丁酯 纳米球 |
| 英文关键词:Giant Panda, Ailuropoda melanoleuca Interferon-gamma Polybutylcyanoacrylate Nanosphere |
| 基金项目:四川省科技计划项目（No. 2012JQ0037） |
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| 中文摘要: |
| 为了制备并评价大熊猫（Ailuropoda melanoleuca）重组干扰素γ（IFN-γ）聚氰基丙烯酸正丁酯（PBCA）纳米球，本研究利用蛋白质原核表达技术，获得了重组大熊猫IFN-γ蛋白，然后以PBCA为载药材料，采用乳化聚合法制备了大熊猫干扰素γ纳米微球（IFNγ-PBCA-NS），最后借助感染大熊猫流感病毒A/Panda/Sichuan/01/2011（H1N1）的昆明小鼠（Mus musculus）模型，通过灌胃和皮下注射药物初步评价了IFNγ-PBCA-NS的药效。结果表明，大熊猫IFN-γ的蛋白分子量约为33.5 ku，所制备的大熊猫IFNγ-PBCA-NS外观规整，粒径在50 ~ 200 nm之间，跨距0.55，大小较均匀，包封率为56.7%，载药量为0.86%。灌胃和注射两种给药途径中，IFNγ-PBCA-NS组小鼠的生命延长率均显著高于IFN-γ组（P < 0.05或P < 0.01）。显示IFNγ-PBCA-NS在小鼠体内有更好的缓释和抗病毒作用，可为进一步研究制备大熊猫多肽类药物微粒提供参考。 |
| 英文摘要: |
| To prepare and investigate the recombinant Interferon-gamma (IFN-γ) nanosphere loaded in polybutylcyanoacrylate (PBCA) in Giant Panda (Ailuropoda melanoleuca), we firstly obtained the recombinant IFN-γ protein through prokaryotic protein expression technology, and then, by choosing PBCA as the drug delivery material, prepared the INF-γ PBCA nanosphere (IFNγ-PBCA-NS) using an emulsion polymerization method. Further more, the Kunming Mice (Mus musculus), which were infected with giant panda influenza virus A/Panda/Sichuan/01/2011 (H1N1), were used as models to test the efficacy of IFNγ-PBCA-NS compared to that of IFNγ after intragastric administration and subcutaneous injection of drugs. The results demonstrated that the molecular weight of INF-γ protein was about 33.5 ku, and the IFNγ-PBCA-NS was globular in appearance, with uniform particle size. The diameters ranged from 50 to 200 nm, the span was 0.55, the encapsulation efficiency of the particles was 56.7% and the drug loading was 0.86%. Moreover, the prolonged life rate of mice in the IFNγ-PBCA-NS group was significantly higher than that of the IFNγ group in both intragastric administration and subcutaneous injection approaches (P < 0.05 or P < 0.01). In summary, IFNγ-PBCA-NS showed better sustained release and antiviral activity in mice, which could provide references for further research on the preparation of the Giant Panda peptide drug microparticles. |
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