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边高鹏,焦海华,明洁.2015.苯胺对黑斑蛙蝌蚪外周血红细胞的遗传毒性效应.动物学杂志,50(6):886-894.
苯胺对黑斑蛙蝌蚪外周血红细胞的遗传毒性效应
Genotoxic Effects of Aniline on Peripheral Blood Erythrocytes in Black-spotted Pond Frog (Rana nigromaculata) Tadpoles
投稿时间:2015-01-27  修订日期:2015-10-30
DOI:DOI: 10.13859/j.cjz.201506008
中文关键词:  苯胺  微核试验  彗星试验  黑斑蛙蝌蚪  遗传毒性
英文关键词:Aniline  Micronucleus test  Comet test  Rana nigromaculata tadpoles  Genotoxicity
基金项目:中国科学院环境生物技术重点实验室开放研究基金(EBT2013A001),长治学院科研项目(2010112)
作者单位E-mail
边高鹏 长治学院生物科学与技术系 biangaopeng@163.com 
焦海华 长治学院生物科学与技术系  
明洁 西北农林科技大学林学院  
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中文摘要:
      为从不同遗传终点检测苯胺对黑斑蛙蝌蚪红细胞的遗传毒性,将黑斑蛙蝌蚪暴露于0、3.45、17.25、34.50、69.0 μg/L不同浓度苯胺96 h后,采用微核试验测定红细胞微核率,通过彗星试验测定彗星尾长和尾距的变化。微核试验结果显示:各浓度处理组微核率均显著高于空白对照组,但由于苯胺所致的细胞破裂和Heinz小体的影响,微核率和浓度之间并未出现明显的浓度-效应关系。彗星试验结果显示:不同浓度苯胺处理组与空白对照组相比,蝌蚪红细胞尾长和尾距均显著增加并与处理浓度之间存在显著的浓度-效应关系。上述结果表明,苯胺可诱发黑斑蛙蝌蚪红细胞的染色体、DNA损伤,具有较强的遗传毒性效应;苯胺最高浓度处理组69.0 μg/L蝌蚪红细胞DNA损伤水平与5 mg/L环磷酰胺相近,显现明显的DNA损伤,因此建议渔业水质标准对水体中苯胺限量的规定不应高于此值。
英文摘要:
      To explore the genotoxicity of aniline to the erythrocyte of Rana nigromaculata tadpoles from different genetic endpoints, the tadpoles were exposed to four concentrations (0, 3.45, 17.25, 34.50 and 69.0 μg/L) of aniline for 96 h. Micronuclei frequencies was measured by micronucleus test, the tail length and the tail moment were assessed by comet assay. The values obtained were statistically analyzed using computer software Statistical Package for Social Sciences (SPSS) version 17.0. One-way analysis of variance (ANOVA) followed by Duncan, s multiple range test was used to analyze the data. Significance was established at P<0.01 level. The results of micronucleus test showed that the erythrocyte micronuclei frequencies of all treatment groups were significantly higher than that of the normal group (Fig. 1, Table 2), but as a result of the influence of cell rupture (Table 1) and Heinz corpuscle caused by aniline, there wasn’t obvious concentration-effect relation between micronucleus frequency and the concentrations of anline. The results of comet assay showed that both the tail length and the tail moment of all treatment groups were significantly higher than the control group (Fig. 2, Table 3), moreover the two indexes increased in a dose-dependent manner with the rise of the aniline concentration. The results aforementioned suggested that aniline induced chromosomes and DNA damage in erythrocytes of Rana nigromaculat tadpoles, indicated stronger genetic toxic effect. Furthermore, the level of DNA damage at the highest concentration (69.0 μg/L) was similar to that of the positive control (5 mg/L cyclophosphamide), therefore there's a recommendation that the restriction of aniline in water body constituted by water quality standard for fisheries should not be higher than the value.
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