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陈文明,何敏,梁晓霞,汪开毓,方静,陈正礼,杨欢,李雪.2015.Ghrelin在成年中国黄羽鹌鹑消化道中的定位.动物学杂志,50(4):628-634.
Ghrelin在成年中国黄羽鹌鹑消化道中的定位
Identification of Ghrelin Cells in Digestive Tract of Adult Chinese Yellow Quail, Coturnix japonica
投稿时间:2014-10-21  修订日期:2015-06-23
DOI:DOI: 10.13859/j.cjz.201504016
中文关键词:  中国黄羽鹌鹑,消化道,Ghrelin,免疫组织化学
英文关键词:Chinese Yellow Quail(Coturnix japonica)  Digestive tract  Ghrelin  Immunohistochemistry
基金项目:四川省教育厅科研项目(No. 13SZA0249, 10ZB035),四川省科技厅应用基础计划项目(No. 2014JY0113)
作者单位E-mail
陈文明 四川农业大学动物医学院 0goldendream0@sina.com 
何敏 四川农业大学动物医学院 hemin09@163.com 
梁晓霞 四川农业大学动物医学院  
汪开毓 四川农业大学动物医学院  
方静 四川农业大学动物医学院  
陈正礼 四川农业大学动物医学院  
杨欢 四川农业大学动物医学院  
李雪 四川农业大学动物医学院  
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中文摘要:
      为探讨Ghrelin免疫反应阳性细胞在成年中国黄羽鹌鹑(Coturnix japonica)消化道的分布规律及形态学特点。应用免疫组织化学SABC法对成年中国黄羽鹌鹑消化道各段Ghrelin阳性细胞进行定位和形态学研究;利用SPSS 17.0软件,对所得数据进行单因素Dunnett多重比较分析。结果显示,Ghrelin阳性细胞在成年中国黄羽鹌鹑腺胃、十二指肠、空肠、回肠和盲肠均有分布,其中在腺胃分布密度最高,为30.70±6.50,盲肠最低,为1.70±1.56,分布密度从腺胃至盲肠呈逐渐降低的趋势。Ghrelin阳性细胞主要分布于腺胃腺叶细胞、肠道黏膜上皮细胞、肠腺上皮细胞和肠固有层之间,细胞形态多为球形、长柱状和三角形。上述结果说明,Ghrelin阳性细胞在成年中国黄羽鹌鹑消化道分布广泛,根据其细胞形态推测中国黄羽鹌鹑消化道Ghrelin阳性细胞可能具有内分泌和外分泌功能。
英文摘要:
      The aim of this study was to investigate distribution regularities and morphological characteristics of the Ghrelin immunoreactive cells in the digestive tract of adult Chinese Yellow Quail(Coturnix japonica). Ten Chinese Yellow Quail (five male and five female) which age were 9 weeks were killed by jugular vein blood, and the every segment of the digestive tract were immediately fixed in neutral formalin. After fixation for 24 h, tissues were dehydrated, paraffin-embedded, sectioned into 5 μm slices. Sections were stained with Immunohistochemistry SABC. The number of Ghrelin positive cells was evaluated in the every segment of the digestive tract using Nikon image analysis software, For each segment of the digestive tract, ten random fields (at ×400 magnification) were quantified, respectively. Statistical analyses were performed using one-way analysis of variance, and Dunnett’s test was employed for multiple comparisons (SPSS 17.0 software). The results showed that Ghrelin positive cells were observed in the proventriculus, duodenum, jejunum, ileum and cecum of Chinese yellow quail(Fig.1).Their distribution density were 30.70±6.50, 26.90±5.10, 25.30±4.27, 11.40±4.14 and 1.70 + 1.56, respectively. There was a trend of gradually decreasing from the proventriculus to the cecum. Ghrelin positive cells were mainly distributed in the proventriculus leaves, intestinal epithelial cells, intestinal glandular epithelium and intestinal lamina propria. The shapes of Ghrelin positive cells were spherical, long columnar and triangle. In conclusion, Ghrelin positive cells were widely distributed in the digestive tract of adult Chinese Yellow Quail. According to the morphology of Ghrelin positive cells in the digestive tract Chinese Yellow Quail, the Ghrelin positive cells might have exocrine and endocrine functions.
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