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傅丽容,徐彤津,史海涛.2015.酵母多糖对中华条颈龟非特异性免疫机能的影响.动物学杂志,50(5):752-757.
酵母多糖对中华条颈龟非特异性免疫机能的影响
Effect of Yeast Polysaccharide on Nonspecific Immune Response of Chinese Striped-neck Turtle (Mauremys sinensis)
投稿时间:2014-09-13  修订日期:2015-06-22
DOI:DOI: 10.13859/j.cjz.201505011
中文关键词:  酵母多糖  中华条颈龟  非特异性免疫机能
英文关键词:yeast polysaccharide  Mauremys sinensis  nonspecific immune response
基金项目:国家自然科学基金面上项目(31372228);海南省自然科学基金资助项目(314078);
作者单位E-mail
傅丽容 海南师范大学生命科学学院 flr@hainnu.edu.cn 
徐彤津 海南师范大学生命科学学院  
史海涛 海南师范大学生命科学学院 haitao-shi@263.net 
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中文摘要:
      本研究旨在探讨饲料中添加不同水平的酵母多糖对中华条颈龟(Mauremys sinensis)非特异性免疫机能的影响。选取体重为134.7±19.1 g的中华条颈龟,随机分成Ⅰ、Ⅱ、Ⅲ、Ⅳ四个组,各组饲料中酵母多糖的添加量分别为每千克龟重0 mg、800 mg、1200 mg和1600mg,并在第15d、30d检测各组免疫器官指数、白细胞数目、血清中溶菌酶及补体C3、C4活性等免疫指标。结果表明,各实验组脾脏指数和肝脏指数与对照组之间差异均不显著(p>0.05);各实验组白细胞数目相比对照组显著增多(p<0.05);在第15d血清中溶菌酶和C3、C4活性均显著高于对照组(p <0.05),在添加量为1200 mg/kg时活性最高,在第30d 各实验组溶菌酶和补体C4活性与同剂量组相比显著下降,补体C4活性与对照组差异不显著(p>0.05)。由此得出,饲料中添加酵母多糖可提升中华条颈龟的非特异性免疫机能,以1200 mg/kg添加水平的效果最佳,且投喂时间不宜过长。
英文摘要:
      This study was designed to evaluate the effects of dietary yeast polysaccharide levels on nonspecific immune response of Chinese Striped-neck Turtle(Mauremys sinensis). The experimental subjects (weight=134.7 ± 19.1 g) were randomly divided into four groups and were fed as follows for 30 days: a commercial diet as control (Ⅰ) and the same diet supplemented with 800 mg/kg (Ⅱ), 1200 mg/kg(Ⅲ), 1600 mg/kg (Ⅳ) yeast polysaccharide. After 15 and 30 days, the immune organ index, number of leucocytes, serum lysozyme and complement C3、C4 activity were detected. The results showed that compared to the control, there was no significant differences in experimental groups for the immune organ index (P >0.05), while the number of leucocytes and lysozyme activity were significantly increased (P <0.05), and the values were highest in group Ⅲ; After 15 days, the serum C3、C4activity in
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