| 杨淞,颜涛,赵柳兰,符红梅,肖青,蒲粟,杜宗君,何智,严太明.2015.齐口裂腹鱼血细胞的发生.动物学杂志,50(2):231-242. |
| 齐口裂腹鱼血细胞的发生 |
| Morphogenesis of Blood Cell Lineages in Ya-?sh （Schizothorax prenanti） |
| 投稿时间：2014-08-23 修订日期：2015-03-14 |
| DOI：DOI: 10.13859/j.cjz.201502009 |
| 中文关键词: 齐口裂腹鱼 血细胞 发生 过碘酸雪夫氏染色 苏丹黑B染色 |
| 英文关键词:Schizothorax prenanti blood cell morphogenesis periodic acid-schiff (PAS) Sudan Black B(SBB) |
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| 中文摘要: |
| 摘要：为了探讨齐口裂腹鱼（Schizothorax prenanti）血细胞发生发育的模式，采用Wright’s，碘酸雪夫氏(PAS)和苏丹黑B(SBB)染色方法对齐口裂腹鱼的头肾、中肾、脾和肝组织印片进行染色，了解其发生的具体组织和发育的一般过程。结果表明：齐口裂腹鱼血细胞可分为红细胞系、淋巴细胞系、单核细胞系、粒细胞系和其他细胞，且这些细胞系的发育均经过3个阶段，即原始阶段、幼稚阶段、成熟阶段。通过观察和统计不同阶段各种血细胞的形态、大小、比例及染色特征，发现原始阶段的血细胞体积较大，其中原始单核细胞最大，大小为（18.90±1.59）μm×（16.32±0.70）μm，在4种组织中原始阶段的红细胞和单核细胞在头肾中所占比例最大，分别为0.64%和0.59%，原粒细胞和原淋巴细胞分别在中肾和脾中比例最大，在肝中少量存在，此外在各组织印片中均发现血栓细胞的存在，在肝中发现巨噬细胞。因此头肾、中肾和脾是齐口裂腹鱼的主要造血组织，少量细胞在肝中产生。粒细胞发育过程中，除原始粒细胞PAS染色阴性外，其他阶段均呈阳性，且阳性随不断成熟逐渐增强；单核细胞从原单核细胞到成熟的单核细胞，PAS阳性逐渐增强；而SBB染色发现，粒细胞、淋巴细胞及单核细胞均呈阳性，未成熟的细胞染色程度不一致，成熟的细胞阳性染色最为强烈。在红细胞系发育过程中经历了细胞由大变小再变大的过程，而粒细胞和淋巴细胞发育过程仅出现由大变小的过程，在红细胞的发育过程中还出现了染色质固缩和血红蛋白增加。 |
| 英文摘要: |
| Abstract: In order to clarify the possible patterns of haemopoiesis in Ya-fish (Schizothorax prenanti), the method of Wright’s, Periodic acid-schiff (PAS) and Sudan back B (SBB) staining were used in the head kidney, trunk kidney, spleen and liver imprints.The present study were helpful to understand the location and process of haemopoiesis in Ya-fish.The data analysis was performed by the software of Microsoft Excel 2010 and all shown as mean±SD.(standard deviation).The results showed that haemopoiesis had the following series: erythropoiesis, granulopoiesis, monopoiesis and lymphopoiesis, consisted of precursor, young and mature stages. According to the morphology, size and proportions of blood cell lineages, monoblasts were the largest in the one of precursors and the size was about (18.90±1.59) μm× (16.32±0.70)μm(Table.1). Proerythroblasts and monoblasts were the predominant cells in head kidney, accounting for 0.64% and 0.59%, respectively. Granuloblasts and lymphoblasts were regularly present in trunk kidney and spleen, rare in liver(Table.1).Furthermore, thrombocytes were observed in four tissues imprints and macrophagocyte could be discovered in liver(Fig.1). So haemopoietic tissue was found mainly in head kidney, trunk kidney and spleen and a small amount occurred in liver. In the granulocytes lineage, except the blast cells, the young and mature cells were PAS-positive; with the maturation process of monocytes, staining intensity of PAS-positive was increased; lymphocytes, granulocytes and monocytes were SBB-positive, and there was a difference of staining intensity in immature stage cells, the mature cell stained intensely with SBB(Fig.2).During maturation process of erythropoiesis, two trends in size were distinguished:two decreasing and one increasing; otherwise gradual chromatin condensation and the increase in haemoglobin content were observed.The morphological changes of granulopoietic and lymphopoiesis were a constant decrease in size during maturation(Fig.3). |
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