| 孟茹,安晶晶,俞诗源,袁静,魏娉婷.2015.妊娠及哺乳期母鼠注射麻黄素对仔鼠肝组织TGF-β1和c-Fos表达的影响.动物学杂志,50(3):352-358. |
| 妊娠及哺乳期母鼠注射麻黄素对仔鼠肝组织TGF-β1和c-Fos表达的影响 |
| Effects of Ephedrine Injection during Pregnancy and Lactation on TGF-β1 and c-Fos Expression in the Liver of Filial Mice |
| 投稿时间：2014-07-18 修订日期：2015-04-13 |
| DOI：DOI: 10.13859/j.cjz.201503004 |
| 中文关键词: 麻黄素 仔鼠 肝脏 TGF-β c-Fos 免疫组织化学 |
| 英文关键词:Ephedrine filial mice liver TGF-β c-Fos immunohistochemisty |
| 基金项目:甘肃省自然科学基金资助项目（No. 1107RJZA141），兰州市社会发展项目（No.2013-3-72） |
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| 中文摘要: |
| 为探讨妊娠及哺乳期小鼠（Mus musculus）注射麻黄素后对仔鼠肝组织TGF-β1及c-Fos表达的影响，将30例受孕小鼠随机分为对照组和麻黄素组。麻黄素组小鼠从受孕第3 d开始连续腹腔注射6.0 g/L麻黄素溶液直到分娩后15 d，每天注射2次，每次0.2 ml；对照组每天2次注射等量的生理盐水。称量检测仔鼠体重和肝重的变化，同时用免疫组织化学方法检测仔鼠肝组织中细胞生长因子TGF-β1和原癌基因c-Fos蛋白表达的变化。结果显示麻黄素组仔鼠体重与对照组相比显著降低（P<0.05或P<0.01），肝体比与对照组相比明显升高（P<0.05或P<0.01），仔鼠肝组织中TGF-β1、c-Fos蛋白阳性表达强度与对照组相比有不同程度的增强（P<0.05或P<0.01），表明妊娠及哺乳期小鼠注射麻黄素影响仔鼠肝脏的发育。 |
| 英文摘要: |
| To investigate the effects on TGF-β1 and c-Fos expression of Mus musculus liver after injecting ephedrine in mice during pregnancy and lactation．30 pregnant mice were randomly divided into control group and ephedrine group．The pregnant mice of ephedrine group continuously intraperitoneal was injected 6.0 g/L ephedrine solution 0.2 ml from becoming pregnant 3 d to until 15 days after delivery，twice a day，the control group was injected with same amount of saline．Changes of body weight and liver weight were detected and the expression of c-Fos protein and TGF-β1 were measured by immunohistochemistry in liver of filial mice．The results showed that the body weight in ephedrine groups was lower than the control groups at the developmental period (Figure 1)(P < 0.05 or P < 0.01)．The ratio of liver and body weight in the ephedrine group was significantly increased than the control group (Figure 2)(P < 0.05 or P < 0.01)．The optical density of TGF-β1 and c-Fos protein expression of liver in ephedrine group was significantly increased than that of the control group at the each developmental period(Figure 3，4，5)(P < 0.01 or P < 0.05)．The results reveal that ephedrine affects the development of filial mice liver in mice injecting ephedrine during pregnancy and lactation． |
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