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周芸芸,朵海瑞,薛亚东,李迪强,冯金朝,张于光.2015.雪豹的微卫星DNA遗传多样性.动物学杂志,50(2):161-168.
雪豹的微卫星DNA遗传多样性
Genetic Diversity Analysis of Microsatellite DNA in Panthera uncia
投稿时间:2014-05-14  修订日期:2015-02-11
DOI:DOI: 10.13859/j.cjz.201502001
中文关键词:  雪豹(Panthera uncia)  非损伤性DNA  微卫星DNA  遗传多样性  遗传结构
英文关键词:Snow leopard (Panthera uncia)  Non-invasive DNA technology  Microsatellite DNA  Genetic diversity  Genetic structure
基金项目:自然保护区生物标本资源共享子平台项目(2005DKA21404)
作者单位E-mail
周芸芸 中央民族大学生命与环境科学学院 zyycici@163.com 
朵海瑞 北京林业大学自然保护区学院  
薛亚东 中国林业科学研究院森林生态环境与保护研究所  
李迪强 中国林业科学研究院森林生态环境与保护研究所  
冯金朝 中央民族大学生命与环境科学学院  
张于光 中国林业科学研究院森林生态环境与保护研究所 yugzhang@sina.com.cn 
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中文摘要:
      利用8个微卫星DNA标记,对来自青海囊谦县、治多县以及甘肃阿克塞县等三个样地的的36份雪豹样品进行遗传多样性及遗传结构研究。结果显示,在8个微卫星位点上共检测到57个等位基因,平均每个位点7.130 个等位基因,有效等位基因数为2.190~5.488,基因频率分布不均匀,期望杂合度为0.543~0.847,平均0.759,多态信息含量为0.458~0.829,平均0.722,表明这8个微卫星位点在研究群体中均为高度多态性位点,显示出较丰富的遗传多样性。分布在青海地区的两个雪豹居群遗传距离较其分别与甘肃省阿克塞县居群小,三个样地雪豹居群之间的遗传距离与地理距离相关;平均遗传分化度Fst(0.053),平均基因流(4.488)以及来自Structure软件的种群结构图推测三个雪豹居群间虽然有一定的遗传距离,但均来自同一个种群,暂无分化现象。
英文摘要:
      The Snow Leopard (Panthera uncia) is a globally endangered and threatened species, and studies on them have been hampered due to their unique ecological characteristics and remote habitat. Eight microsatellite DNA loci were used to analyze the genetic diversity and genetic structure of 36 identified snow leopard individuals from three areas including Nangqian (Qinghai), Zhiduo (Qinghai) and Akesai (Gansu). 57 alleles were detected in 8 microsatellite loci, and the mean number of alleles (A) was 7.13. The effective number of alleles (Ne) ranged from 2.190 to 5.488, with uneven Allelic frequency distribution. The expected heterozygosity (He) ranged from 0.543 to 0.847, with an average of 0.759. And the polymorphism information content (PIC) ranged from 0.458 to 0.829, with an average of 0.722. In summary, the selected microsatellite DNA markers were highly polymorphic loci with relatively rich genetic diversity in these snow leopard populations. It is smaller genetic distance of two snow leopards’ groups in Qinghai than those of groups in Qinghai respectively with the group in Akesai (Gansu). The index of Fst (0.053), the index of Nm (4.488) and the result from the Structure software showed that such 3 snow leopards’ groups haven’t been divided and should be viewed as one population.
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