| 甘远迪,赵金良,Jeerawat Thammaratsuntorn,李传阳.2014.萨罗罗非鱼AQP3 cDNA序列克隆及盐度胁迫下组织表达特征.动物学杂志,49(4):560-569. |
| 萨罗罗非鱼AQP3 cDNA序列克隆及盐度胁迫下组织表达特征 |
| cDNA Cloning of Aquaporin 3 in Sarotherodon melanothern and its tissue expression patterns under salinity stresses |
| 投稿时间：2013-10-17 修订日期：2014-04-29 |
| DOI：10.13859/j.cjz.201404013 |
| 中文关键词: 萨罗罗非鱼 水通道蛋白3 cDNA 组织表达 盐度胁迫 |
| 英文关键词:Sarotherodon melanothern Aquaporin 3 cDNA Tissue expression Salinity stress |
| 基金项目:现代农业产业技术体系专项(No. CARS-49-4B)，上海高校知识服务平台上海海洋大学水产动物遗传育种中心项目(No. ZF1206) |
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| 中文摘要: |
| 利用cDNA末端快速扩增法(RACE)克隆了萨罗罗非鱼Sarotherodon melanothern鳃组织中水通道蛋白AQP3的cDNA序列。AQP3 cDNA全长1894 bp,其中,开放阅读框(ORF)912 bp,编码303个氨基酸,5’和3’非编码区长度分别为98bp和884bp。氨基酸序列分析显示,萨罗罗非鱼AQP3与莫桑比克罗非鱼(Oreochromis mossambicus)同源性最高,达94%。应用实时荧光定量PCR技术(qRT-PCR)检测了不同盐度胁迫下萨罗罗非鱼11种组织中AQP3 mRNA的相对表达水平。0、15盐度下,鳃、肌肉、皮肤中表达水平相对较高,其他组织表达相对较低,且15盐度中各组织的表达水平低于0盐度；30盐度下,各组织以肠道表达量相对最高。推测在不同的渗透压调节作用中,萨罗罗非鱼是通过不同组织器官中AQP3来参与水的转运过程。 |
| 英文摘要: |
| The complete cDNA sequences of Aquaporin 3 (AQP3) from gills of Sarotherodon melanothern was cloned using rapid amplification of cDNA ends (RACE). The full length of AQP3 cDNA was 1894 bp, containing 912 bp open reading frame and encoding 303 amino acids, with a 98 bp 5’ untranslated regions (UTR) and 884 bp 3’-UTR. The amino acid sequence comparison showed that AQP3 of S. melanothern shared the highest identity with Oreochromis mossambicus (94%). Relative tissue expression of AQP3 mRNA under different salinity stresses were estimated by real-time quantitative PCR. The higher expression were detected in skin, gill and muscle than in other tissues at 0 and 15 salinity, moreover, the expression level in 15 salinity was lower than that in freshwater in all tissues. The expression level was higher in intestine than in other tissues at 30 salinity. The results suggested S. melanothern could utilize AQP3 in different tissue to respond to water transfer under different osmoregulation process. |
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