| 蔡雷,王浩,王文波,戴振东,石爱菊.2014.鸽子慢性电刺激用电极转接装置及其固定方法.动物学杂志,49(2):280-285. |
| 鸽子慢性电刺激用电极转接装置及其固定方法 |
| Design and Application of an Electrode Adapter for Chronic Experiments in Pigeon |
| 投稿时间：2013-08-07 修订日期：2014-03-03 |
| DOI： |
| 中文关键词: 鸽子 电刺激 电极转接装置 无线刺激器 运动诱导 |
| 英文关键词:Pigeon (Columba livia) electrical stimulation Electrode adapter Wireless stimulator Movement induction |
| 基金项目:国家自然科学基金面上项目(No. 61375096，61175105), 高等学校博士学科点专项科研基金项目(No. 20133218120038), 中国博士后科学基金特别资助项目(No. 2012T50497) |
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| 中文摘要: |
| 慢性电极植入以及无线刺激技术被广泛应用于动物自由活动状态下的脑区功能研究。实现刺激器与脑内植入电极过渡连接的转接装置需固定在颅骨之上。鸟类特殊的骨质构造不利于转接装置的长期固定。以鸽子(Columba livia)为例设计制作了一种用于慢性运动诱导实验的9通道电极转接装置, 长12.8mm、宽9.5mm、高5.5mm, 重0.42g; 根据鸽子颅骨特点在固定过程中对颅骨表面进行粗糙化处理增加固定时与牙科水泥的接触面积, 并选取特定位点拧入螺钉进行固定, 有效延长了转接装置的固定时间。经实验验证能够在鸽子头部稳定固定6个月以上, 满足鸽子长期运动诱导研究的需求, 未对动物正常活动产生影响。该装置及其固定方法亦可为其他小型动物的脑区功能研究提供借鉴。 |
| 英文摘要: |
| Electrode implantation and wireless stimulation are widely used in investigating brain functions in conscious, freely moving animals. Electrode adapter connecting the wireless stimulator and the implanted electrode needs to be fixed upon the skull. The specialization in bird bone makes it difficult for long-term fixation of the adapter. Here describes a 9-channel electrode adapter for chronic movement induction in the pigeon (Columba livia). It is 12.8mm long, 9.5mm wide, 5.5mm high, and weights 0.42g. According to the characteristics of pigeon's skull, we roughen the skull surface, upon which the electrode adapter is mounted, by means of drilling a series of pits. This process forms a few “contact cones” to increase the contact area between the skull and the electrode adapter when binding by the dental cement. Additionally, three micro-screws are used to reinforce the binding at the specific point of the skull. These processes effectively extend the duration of the adapter on pigeon's head. The preliminary experiments have shown that all the tested adapters (n=6) are fixed on the skull stably for at least 6 months without influence on pigeon's normal activities. The adapter and its fixation method are suitable for long term investigation of brain functions in freely moving birds and would provide inspiration for other animal studies. |
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