| 龚旭东,田英芳,梁刚.2014.水温与雌二醇对中国大鲵幼体生长与肝的影响.动物学杂志,49(3):376-383. |
| 水温与雌二醇对中国大鲵幼体生长与肝的影响 |
| Water Temperature and Estradiol Influence on the Growth and Liver of the Young Chinese Giant Salamander Andrias davidianus |
| 投稿时间：2013-07-08 修订日期：2014-04-28 |
| DOI：10.13859/j.cjz.201403009 |
| 中文关键词: 中国大鲵幼体 水温 17β-雌二醇 生长 肝 |
| 英文关键词:Young Andrias davidianus Water temperature 17β-Estradiol Growth Liver |
| 基金项目:陕西省水利科技计划项目（No.2012-2-21） |
|
| 摘要点击次数: 2506 |
| 全文下载次数: 2107 |
| 中文摘要: |
| 为探讨水温与外源17β-雌二醇(E2)对中国大鲵(Andrias davidianus)幼体生长的影响,设置了3个水温梯度、2个E2浓度梯度与3个时间梯度交互处理共18组,同时设置了3个水温梯度组。幼鲵每天用E2暴露4 h,分别在出膜143 d、182 d、248 d且每次喂食5 h后,分别称量各组幼鲵的体重。为进一步探寻E2对幼鲵生长影响的原因,采用一般组织学技术对出膜143 d幼鲵肝的组织结构进行了观察。结果表明,幼鲵在(20?1)℃水温生长最快、在(13?1)℃生长次之、在自然河道水生长最慢；在(20?1)℃水温下,外源E2暴露对幼鲵生长具有一定的抑制作用,且E2暴露时间越长、抑制作用越强,暴露剂量越大、抑制作用越大；E2暴露浓度为25 μg/L的幼鲵极少数肝细胞质内出现了脂肪空泡,肝血窦有所扩大；E2暴露浓度为250 μg/L的幼鲵约30%肝细胞与细胞核出现肿大、细胞质中出现脂肪空泡、被覆在肝实质表面的上皮细胞向肝实质内迁移、肝血窦扩大等病理现象。本文认为,外源E2暴露对幼鲵肝组织结构造成的损伤,是抑制幼鲵生长的主要原因。 |
| 英文摘要: |
| To explore water temperature and 17β-estradiol (E2) influence on the growth of the young Chinese giant salamander Andrias davidianus, 18 groups were formed of factors interaction among 3 water temperature、2 E2 concentration and 3 exposure time, and added 3 water temperature groups as control. The weight of the young Andrias davidianus, which post-hatching 143 d、182 d、248 d fed after 5 hours, was calculated, when exposed E2 for 4 hour per days. The liver microstructure was observed in young Andrias davidianus post-hatching 143 d using histological method, in order to reveal the mechanism of E2 effect on liver. The results showed the growth of the young Andrias davidianus is fast at (20?1)℃, less at (13?1)℃, slowest at natural river water temperature. E2 have adverse effects on growth of the young Andrias davidianus at (20?1)℃. In addition, the toxity of E2 increased with the exposure time and E2 concentration increase. Vacuolization in hepatic cell and enlargement in hepatic sinusoid are observed in the liver of the young Andrias davidianus at 25 μg/L E2. Moreover, epithelium cell in the liver capsule migrated into the inner tissue at 250 μg/L E2. Growth of the young Andrias davidianus was inhibited attributed to liver damage, which caused by the E2. |
| 附件 |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
